利用酵母双杂合系统筛选与hERRα1相互作用的蛋白质  

Identification of ERRα1-interacting proteins by yeast two-hybrid screening

作　　者：李渝萍[1] 陈敏[1] 陈彬[1] 陈健[1] 李强[1] 周度金[1] 

机构地区：[1]第三军医大学基础医学部生物化学与分子生物学教研室,重庆400038

出　　处：《第三军医大学学报》2003年第15期1352-1354,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金"两个基地建设"专项资助项目 ( 9910 76 194 6 ) ;国家自然科学基金资助项目 ( 30 0 70 359)

摘　　要：目的　利用酵母双杂合系统筛选乳腺组织中与人雌激素受体相关受体α1(Humanestrogenreceptorrelated receptorα1,hERRα1)相互作用的蛋白质 ,特别是新的核受体辅助活化因子 ,以进一步研究核受体辅助活化因子在乳腺癌发生、发展中如何参与ERR1调控基因的表达。方法　以质粒pGBT9 hERRα1 LBD为“诱饵” ,筛选人乳腺组织cDNA文库。结果　筛得有阳性相互作用的克隆共 17个。除 3种为已知核受体辅助调节因子外 ,其他为功能已经明确的蛋白 ,其中有3种与hERRα1有明显相互作用。结论　核受体辅助活化因子SRC 1,RIP14 0 。Objective To understand the role and the regulatory mechanism of hERRα1 in breast cancer development by means of cloning the genes which encode the hERRα1 interacting proteins by screening a mammary gland cDNA expression library using yeast two hybrid approach. Methods hERRα1/LBD was used as the bait to screen a human mammary gland cDNA expression library by yeast two hybrid approach. Results A total of 17 positive clones were obtained and analyzed. Except 3 known nuclear receptor coregulators, the rest were known proteins and 3 of them interacted with hERRα1 strongly. Conclusion Nuclear receptor coregulators SRC 1, RIP140, TRIP and some known proteins can interact with orphan nuclear receptor hERRα1 in human mammary gland tissue.

关 键 词：人雌激素受体相关受体α1 核受体辅助活化因子 酵母双杂合系统 乳腺癌 

分 类 号：Q51[生物学—生物化学] Q71