生长抑素抑制多器官功能衰竭时肠黏膜肥大细胞活性  

Somatostatin suppressed the activity of intestinal mucosal mast cells in rats with multiple organ failure

作　　者：唐承薇[1] 蓝程[2] 

机构地区：[1]四川大学华西医院消化内科,成都610041 [2]海南省人民医院消化内科

出　　处：《中华消化杂志》2003年第8期461-465,共5页Chinese Journal of Digestion

基　　金：国家杰出青年科学基金资助项目 (3 972 5 0 12 )

摘　　要：目的　探讨生长抑素 (SST)对多器官功能衰竭 (MOF)时肠黏膜肥大细胞 (IMMC)活性的调节及其病理生理意义。方法　酵母多糖腹腔注射法制作大鼠MOF模型 ,注射酵母多糖后 ,经尾静脉输入SST(剂量为 2 .30 0ng·kg-1·h-1和 0 .0 2 3ng·kg-1·h-1) ,观察动物肠、肝、肾、肺、心等重要器官的组织病理学改变及丙氨酸转氨酶 (ALT)、肌酐 (Cr)、氧分压 (PO2 )等功能指标变化 ,测定动物外周血和小肠组织组胺及肿瘤坏坏死因子 α(TNF α)水平 ,透射电镜观察IMMC超微结构变化。结果　以 2 .30 0ng·kg-1·h-1输注SST后 ,与MOF对照组相比 ,大鼠各重要器官炎症病变明显减轻。肝功能明显恢复 ,ALT下降 5 3% ;肾脏清除功能提高 ,血Cr下降 6 0 % ;肺通气改善 ,血PO2 升高 5 0 % ;同时MOF大鼠外周血组胺水平无明显变化 (P >0 .0 5 ) ;小肠组织组胺水平由 (8.6 0± 0 .5 0 )ng/g蛋白升高至 (14 .5 0± 1.0 8)ng/ g蛋白 (P <0 .0 1) ;IMMC脱颗粒现象明显改善。小肠组织TNF α水平较MOF对照组显著降低 (P <0 .0 1)。结论　SST可通过抑制IMMC脱颗粒 ,增强其稳定性 ,从而改善MOF病变 ,阻止MOF发生发展。Objective To investigate the effect of somatostatin(SST) on the activity of intestinal mucosal mast cells(IMMC) and its pathological significance in the development of multiple organ failure(MOF). Methods The rat model of MOF was established by the peritoneal injection of zymosan. Thirty minutes after the injection of zymosan, SST at 2.300 ng·kg -1 ·h -1 or 0.023 ng·kg -1 ·h -1 was injected respectively through tail veins. The concentration of histamine and tumor necrosis factor-α (TNF-α) in plasma and intestinal tissue were measured. The pathological alterations of essential organ including intestine, liver, kidney, lung and heart were studied under light microscope. Their corresponding functions were reflected with alanine aminotransferase (ALT), cretinine (Cr) and oxygen pressure (PO 2). In addition, the ultra structure of the IMMC was observed under a transmission electronic microscope. Results Compared with the controlled rats, the rats injected with SST (2.300 ng·kg -1 ·h -1 ) showed less serious inflammatory response under light microscope. ALT and Cr were decreased 53% and 60% respectively. However, the lung ventilation was improved and PO 2 was increased by 50%. The histamine level in the intestinal tissue from rats treated with SST remarkably increased( ( 8.60± 0.50 ) ng/g protein to ( 14.50± 1.08 ) ng/g protein), whilst the plasma histamine level did not show any significant changes. Exogeneous SST also resulted in lower level of TNF-α in intestine but no changes in plasma. Furthermore, degranulation of IMMC from the rats treated with SST was less obvious. Conclusion SST may prevent from or arrest the development of MOF through suppression of the release of inflammatory mediators, such as histamine and TNF-α.

关 键 词：生长抑素 多器官功能衰竭 肠黏膜肥大细胞 活性 组织病理学改变 丙氨酸转氨酶 

分 类 号：R96[医药卫生—药理学]