瑞香素对体外培养恶性疟原虫超氧化物歧化酶活性及DNA合成的影响  

Effect of Daphnetin on SOD Activity and DNA Synthesis of Plasmodium falciparum in vitro

作　　者：牟凌云[1] 王琴美[1] 倪奕昌[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所世界卫生组织疟疾,血吸虫病和丝虫病合作中心,上海200025

出　　处：《中国寄生虫学与寄生虫病杂志》2003年第3期157-159,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金(No.39970669)

摘　　要：目的 研究瑞香素(DPNT)对体外培养恶性疟原虫超氧化物歧化酶(SOD)活性及DNA合成的影响。方法 在恶性疟原虫FCC1株体外培养体系中,以SOD试剂盒检测瑞香素、瑞香素铁盐及去铁胺(DFO)对疟原虫SOD活性的影响。疟原虫培养同步化,利用荧光素Hoechest33258测定在瑞香素和去铁胺作用下疟原虫不同发育阶段(环状体和滋养体)的DNA合成水平。结果 与未加药对照组比较,经瑞香素作用后疟原虫总SOD下降约60％(P<0.01),而相应的经去铁胺作用后,疟原虫的总SOD仅下降约22％(P>0.05)。瑞香素铁螯合能力被遮蔽后,几乎失去对疟原虫SOD的影响。同步培养的滋养体阶段疟原虫,在瑞香素作用下DNA合成率明显低于对照组。结论 在体外瑞香素可显著降低疟原虫SOD活性,并影响滋养体阶段疟原虫的DNA合成。Objective To investigate the effect of daphnetin on superoxide dismutase (SOD) activity and DNA synthesis in P. falciparum in vitro. Methods The effect of daphnetin, daphnetin-Fe complex and desferrioxamine B on SOD activity of P. falciparum (P. f) FOCI in vitro was determined with a SOD test-kit. The level of DNA synthesis of P. f synchronized cultured in vitro at various developmental stages after treatment of daphnetin or desferrioxamine B was assayed by fluorescein Hoechest 33258. Results The total SOD activity decreased by 60% after daphnetin treatment while it only decreased by 22% if treated with desferrioxamine B. No effect on SOD activity of P. f treated with daphnetin-Fe complex was observed. The level of DNA synthesis of P. f trophozoites in synchronized in vitro culture was significantly lower than that of the control. Conclusion Daphnetin lowered SOD activity and decreased DNA synthesis of P. f in vitro.

关 键 词：瑞香素 体外培养 恶性疟原虫 超氧化物歧化酶 活性 DNA 

分 类 号：R382.31[医药卫生—医学寄生虫学]