日本血吸虫门脉内童虫表膜抗原组分及其保护性免疫力研究  

Studies on Fractionation and Protective Immunity of the Membrane Antigen from Hepato-portal Juveniles of Schistosoma japonicum

作　　者：王敏[1] 易新元[2] 曾宪芳[2] 周东明[2] 张顺科[2] 章洁[2] 袁仕善[2] 

机构地区：[1]中南大学湘雅二医院检验科,长沙410011 [2]中南大学湘雅医学院寄生虫学教研室,长沙410078

出　　处：《中国寄生虫学与寄生虫病杂志》2003年第3期147-149,153,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：WHO/TDR资助项目(980255);湖南省科委基金(00jzy 2115)

摘　　要：目的 探讨日本血吸虫门脉内童虫表膜抗原(SjHmAg)的免疫特性,观察其抗日本血吸虫(Sj)的保护效果。方法 用SDS-PAGE电泳技术分析SjHmAg蛋白组分,酶联免疫电转移印迹(EITB)分析感染兔血清(IRS)和正常兔血清(NRS)对SjHmAg的识别;用完整SjHmAg免疫昆明鼠3次,分别在0、2、4周进行,第6周每鼠经腹部感染40±1条Sj尾蚴,42天后剖杀,计数虫数及肝卵数。结果 用SDS-PAGE电泳获得SjHmAg主带7条,IRS主要能识别SjHmAg23、33和63kDa等10个抗原组分;间接ELISA测其抗体滴度>1:6400,与对照组相比,SjHmAg免疫小鼠的减虫率为16.2％,减卵率为55.4％。结论 用SDS-PAGE获得了不同分子量的SjHmAg蛋白,EITB鉴别出具有免疫活性的蛋白分子,且SjHmAg对Sj攻击感染及雌虫生植似有一定的抗性。Objective To explore the immunological characteristics of the membrane antigen from hepato-portal juveniles of Schistosoma japonicum and its protective immunity against S. japonicum (Sj) in mice. Methods Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and enzyme-linked immune electro-transfer blot(EITB) methods were used to recognize the membrane antigens from hepato-portal schistosomula (SjHmAg) by infected rabbit sera (IRS) and normal rabbit sera (MRS). Kunming mice were immunized subcutaneously three times(0,2,4weeks) with SjHmAg. Each mouse was challenged with 40±1 cercariae. Six weeks later the mice were killed, worms and liver eggs were counted. Results 7 major protein bands appeared on SDS-PAGE. IRS mainly reacted specifically with SjHmAg of 23, 33 and 63 kDa. Compared with the control groups, the reduction rate of worms and eggs per gram liver in the experimental group were 16.2% and 54.4 % , respectively. Conclusion Different protein components from SjHmAg are obtained using SDS-PAGE, and the antigen can induce a protective immunity against Sj in mice.

关 键 词：日本血吸虫 门脉内童虫 表膜抗原 保护性免疫力 SDS-PAGE电泳技术 

分 类 号：R383.24[医药卫生—医学寄生虫学]