法呢基焦磷酸合酶基因的克隆及双元载体的构建  

Molecular Cloning and Construction of Plant Expression Vector of Farnesyl Phosphate Synthase Gene from Menthor spicata L

作　　者：崔红[1] 郭小玲[2] 时向东 刘国顺[1] 

机构地区：[1]河南农业大学农业生物技术重点实验室国家烟草栽培生理生化研究基地,河南郑州450002 [2]厦门大学海洋与环境学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2004年第2期253-255,共3页Journal of Xiamen University：Natural Science

基　　金：国家烟草专卖局科技项目(110200101005)资助

摘　　要：以留兰香(MenthaspicataL)为材料,利用RT PCR方法克隆法呢基焦磷酸合酶基因(fps)的cDNA,核酸序列分析表明,该基因的编码区长1050bp,编码349个氨基酸.进一步将fps基因插入植物表达载体BinAR,酶切鉴定及测序结果都证明fps的植物双元表达载体构建成功,为fps基因在烟草中的异源表达奠定了基础.Farnesyl phosphate synthase is a key enzyme in the biosynthesis pathway of sequiterpenoids, which play an important role in tobacco pathogen-resistance response. By RT-PCR technology, the cDNA of fps gene of Menthor spicata L was cloned and sequenced. The result demonstrated that the sequence of coding region was 1 050 bp,encodes a protein of 349 amino acid. In order to transfer fps gene into tobacco, plant expressing vector pBinARfps was constructed .

关 键 词：法呢基焦磷酸合酶基因 克隆 双元载体 载体构建 留兰香 RT-CR方法 异戊二烯途径 烟草 

分 类 号：S572[农业科学—烟草工业] Q785[农业科学—作物学]