氢溴酸槟榔碱对大鼠离体结肠平滑肌细胞作用的研究  

A Study of the Effects of Arecaline Hydrobromide on the Dispersed Colonic Smooth Muscle cells in Rats

作　　者：司春峰[1] 魏睦新[1] 轩原清史 

机构地区：[1]南京医科大学第一附属医院中医科,江苏南京210029 [2]日本HIPEP研究所

出　　处：《上海中医药杂志》2004年第3期48-50,共3页Shanghai Journal of Traditional Chinese Medicine

基　　金：江苏省 1 35工程重点医学人才基金资助 (1 350 38)

摘　　要：为研究氢溴酸槟榔碱 (Ah)对结肠平滑肌细胞运动的影响及其作用机制。制备大鼠游离的结肠平滑肌细胞 ,观察Ah引起的结肠平滑肌细胞收缩效应及其他工具药对收缩效应的影响。结果 :Ah可使流离的单个结肠平滑肌细胞收缩 ,在 1× 10 -6～ 1× 10 -3mol/L的浓度范围内 ,呈剂量依赖性 ,最大效应浓度为 1× 10 -3 mol/L。阿托品可阻断其直接收缩作用 ,酚妥拉明则无这种作用 ;钙鳌合剂EGTA可抑制其直接收缩作用 ,而TMB 8阻断胞内Ca2 + 释放后 ,对这种抑制作用无显著影响。结论 :Ah通过M受体引起结肠平滑肌细胞收缩 ,其收缩作用通过细胞外Ca2 + 的内流介导。To study the kinetogenic effects of Ah on colonic smooth muscle cells of rats and explore the contractile mechanism, smooth muscle cells were isolated from the colon of the rat, and the contractile response of colonic smooth muscle cells to Ah and the effects of other medicines were separately observed. Results: Ah can make dose dependent contraction with the concentration range from 1 10 6 mol/L to 1 10 3 mol/L , the concentration of the maximal response is 1 10 3 mol/L. contraction caused by Ah could be inhibited by Atropin and EGTA, inhibition by Regidine and TMB 8 is not significant. It is showed that the contraction caused by Ah is mediated by M receptor and depends on the presence of extracellular calcium.

关 键 词：氢溴酸槟榔碱(Ah) 促动力作用 平滑肌细胞 结肠 大鼠 

分 类 号：R285.5[医药卫生—中药学]