高效液相色谱法测定银杏叶缓释胶囊中银杏内酯的含量  

Determination of ginkgolides in ginkgo sustained release capsules by HPLC

作　　者：王淑君[1] 彭缨[1] 李钢 陈济民[1] 毕殿洲[1] 

机构地区：[1]沈阳药科大学,辽宁沈阳110016 [2]辽宁康博士制药有限公司,辽宁鞍山114100

出　　处：《中国医院药学杂志》2004年第3期148-150,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的 :建立高效液相色谱法 ,测定银杏叶缓释胶囊中银杏内酯的含量。方法 :色谱柱 :C18(Sperisorb4 .6mm× 2 5 0mm ,5 μm) ,流动相 :甲醇 水 (30∶70 ) ,流速 :1.0mL·min-1,柱温 :2 5℃ ,示差折光检测器。结果 :白果内酯、银杏内酯A、银杏内酯B、银杏内酯C进样量与色谱峰面积在 10～ 80mg·L-1范围内呈良好的线性关系 ,R分别为 0 .9996 ,0 .9995 ,0 .9999,0 .9997,回收率分别为 10 0 .0 % ,99.1% ,99.2 % ,99.9% ,精密度测定RSD小于 6 .6 %。结论 :该方法灵敏、准确、经济 ,可用于测定银杏叶缓释胶囊中银杏内酯的含量。OBJECTIVE An HPLC method was established for the determination ginkgolides in ginkgo sustained release capsules. METHODS Analytical column was Spherisorb C_ 18 ,the mobile phase consisted of methanol-water (30∶70),the flow rate was 1.0 mL·min -1 . The detector was a refractometer. RESULTS The calibration curves of ginkgolides were linear in the concentration range of 10～80 mg·L -1 ( r = 0.9996 , 0.9995 , 0.9999 , 0.9997 ). The recovery was 100.0% . 99.1% , 99.2% , 99.9% , respectively.The RSD was less than 6.6% . CONCLUSION The method was simple, accurate and can be used for determination of ginkgolides in the ginkgo sustained release capsules.

关 键 词：银杏内酯 高效液相色谱法 银杏叶缓释胶囊 

分 类 号：R927.2[医药卫生—药学]