高效液相色谱法测定格列齐特缓释片的含量  

Determination of gliclazide sustained-release tablets by HPLC

作　　者：丁洪亮[1] 张根元[2] 陶伟博[2] 

机构地区：[1]宜兴市人民医院,江苏宜兴214200 [2]江苏省药物研究所,江苏南京210009

出　　处：《中国医院药学杂志》2004年第3期156-157,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的 :建立格列齐特缓释片的含量测定方法 ,以控制该制剂的质量。方法 :采用高效液相色谱法 (HPLC)测定格列齐特缓释片的含量 ,以C18为固定相 ,甲醇 0 .0 2mol·L-1磷酸二氢钠溶液 (6 4∶36 )为流动相 ,检测波长为 2 2 9nm。结果 :格列齐特在 8.0 16～ 12 .0 2 4mg·L-1浓度范围内 ,峰面积与其浓度呈良好的线性关系 ,相关系数r =0 .9992 ,辅料无干扰 ,平均回收率为 99.5 % ,RSD为 0 .7% (n =6 )。结论 :本方法简便、快速、准确 ,可用于格列齐特缓释片的含量测定。OBJECTIVE To establish a method for determining the content of gliclazide sustained-release tablets by HPLC. METHODS A C_ 18 column was used and the mobile phase consisted of methanol-sodium dihydrogen phosphate solution (64∶36).The detection wavelength was 229 nm. RESULTS The linear range of calibration curve of gliclazide was 8.016 ～ 12.024 mg·L -1 ( r = 0.9992 ), and the average recovery was 99.5% , RSD= 0.7% ( n =6). CONCLUSION The method is simple,rapid and accurate. It can be used for determining the content of gliclazide sustained-release tablets.

关 键 词：格列齐特缓释片 高效液相色谱法 含量测定 

分 类 号：R927[医药卫生—药学]