东方拟无枝酸菌糖基转移酶基因gtfE的克隆(英文)  

Cloning of gtfE gene encoding glycosyltransferase from Amycolatopsis orientalis

作　　者：金飞燕[1] 李国亮[1] 王旻[1] 戈梅[2] 陈代杰[2] 

机构地区：[1]中国药科大学,南京210009 [2]上海来益生物药物研究开发中心,上海201203

出　　处：《中国抗生素杂志》2004年第3期134-137,共4页Chinese Journal of Antibiotics

摘　　要：糖基转移酶基因 gtf E位于万古霉素产生菌东方拟无枝酸菌 (Amycolatopsisorientalis)染色体上。通过 PCR的方法并对PCR反应条件进行了优化 ,扩增得到了较高产量的 PCR产物。PCR产物经酶切回收后克隆到 p Bluscript KS+质粒载体 ,转化E.coli DH5 α感受态细胞 ,筛选得到一阳性克隆。测序后与文献报道的基因序列做同源比较 ,结果仅有两个碱基的差异。Glycosyltransferase gene gtfE is a key gene involved in biosynthesis of vancomycin . Through PCR method and optimization of PCR reaction system,the objective PCR product with high yield was obtained. It was ligated into pBluescript Ⅱ KS _ vector and transferred into competent cells of Escherichia coli DH5α. Positive clone was selected and sequenced. The sequencing result showed that there were only two bases different from the reported sequence.

关 键 词：万古霉素 东方拟无枝酸菌 糖基转移酶 基因克隆 

分 类 号：Q781[生物学—分子生物学]