紫外线诱变产黄青霉菌原生质体选育高产菌种  

Breeding the high-productive penicillin strain through mutate protoplast with ultrauiolet

作　　者：唐丽华[1] 

机构地区：[1]哈药集团制药总厂,150086

出　　处：《黑龙江医药》2004年第2期129-131,共3页Heilongjiang Medicine journal

摘　　要：在菌种诱变筛选过程中,往往由于细胞壁的存在而影响其对外界理化因子的敏感性,因而我们采用细胞脱壁后再进行紫外线诱变处理的方法,选育青霉素高产优质菌株.分离纯化得到276#菌株,摇瓶考查青霉素效价和总产量分别提高了8.2%和6.4%,对氧的需求也有所降低,达到了预期效果.During the selecting of fungus'mutation process,frequently sensitivity to the physics and chemistry divisor in the outside world was effect by the cell wall.As a result we adopt mutate protoplast with the ultraviolet ray after leave the cell wall to breeding the high-productive penicillin producing strain.Isolated and extracted product strain 276~#,the result of flask-shaking test showed that the penicillin fermentation and total yield increase 8.2%and 6.4%.The demand to oxygen also cuts down somewhat.Attaining anticipates effect.

关 键 词：紫外线 诱变 产黄青霉菌 原生质体 选育 菌种 

分 类 号：TQ465[化学工程—制药化工]