毛细管等电聚焦/毛细管无胶筛分电泳二维蛋白质分离平台的构建  

Construction of Two-dimensional Separation Platform of Coupling Capillary Isoelectric Focusing with Capillary Non-gel Sieving Electrophoresis for Proteins

作　　者：刘和春[1] 杨春[1] 杨青[1] 张维冰[1] 张玉奎[1] 

机构地区：[1]中国科学院大连化学物理研究所,国家色谱研究分析中心大连116011

出　　处：《分析化学》2004年第3期273-277,共5页Chinese Journal of Analytical Chemistry

基　　金：国家 973人类重大疾病蛋白质组学研究项目 (No .973;0 0 1CB5 10 2 0 2 )

摘　　要：设计并制作了一种新型的中空纤维接口 ,以此为核心构建了毛细管等电聚焦 (CIEF) /毛细管无胶筛分 (CNGE)电泳二维蛋白质分离技术平台 ,实现了将二维凝胶电泳从平板转移到毛细管中。利用该二维分离平台 ,对血红蛋白样品进行了高效、快速分离分析 ,验证了该二维分离平台的可行性及分离效能。实验结果表明 :二维分离系统总的峰个数和分离效能都比各自一维分离系统有较大提高。A novel dialysis hollow fiber interface was designed and fabricated and a two-dimensional separation platform of coupling capillary isoelectric focusing (CIEF) with capillary non-gel sieving electrophoresis (CNGE) for proteins was constructed by the interface. Classic two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) was transferred to the capillary format via the interface. The hemoglobin (Hb) sample was selected to demonstrate the 2-D separation system′s feasibility and resolving power. The 2-D separation system was found to greatly increase the resolving power and overall peak capacity over those obtained for either dimension alone.

关 键 词：蛋白质 二维分离平台 毛细管等电聚焦 毛细管无胶筛分电泳 中空纤维接口 

分 类 号：O658.9[理学—分析化学]