优质蜜环菌的快速培养  

Rapid Cultivation of High Quality of Amillarise Mellea

作　　者：黄燕芬[1] 唐丽[1] 范成五[1] 

机构地区：[1]贵州省农业科学院土壤肥料研究所,贵阳550006

出　　处：《种子》2004年第3期27-29,共3页Seed

摘　　要：本实验采用组织分离法或孢子分离法分离野生菌种 ,加入 1 .2 5 mg/ L四环素的 PDA培养基鉴选、纯化引进菌种两种方式获得优质母种。再用自制木屑培养基扩大繁殖培养二级种 ,青冈嫩枝段培养基培养生产种。初步建立了优质蜜环菌的快速培养技术程序。实验中发现筛选出的母种经木屑培养基培养后 ,再进入生产种培养 ,得到的生产种质量优于母种直接进入枝段培养基培养的 ,且缩小母种用量 1 0倍 ,节约培养基成本约 5 0 %Using the PDA medium with 1.25mg·L -1 acheomycin to authenticate and select the Armillaria Mellea stock culture by bought and separating the organ or the spores from the wild fruiting body. Using one of there two methods can acquire the high quality Armillaria Mellea stock. culture. Then culture it using the self make sawdust medium to become pre culture spawn, and then using sect of the Blue Japanese Oak burgeon medium culture pre culture spawn to become spawn. This trial first step establish the procedure technique process of rapidly culturing the high quality Armillaria Mellea and discover the quality of the spawn which cultured by the self make sawdust medium is exceeding than that directly cultured by sect of the burgeon medium, and reduce the stock dosage 10 times, economize the cost of medium invites 50%.

关 键 词：蜜环菌 快速培养 组织分离法 孢子分离法 培养基 天麻 

分 类 号：S567.239[农业科学—中草药栽培]