乙酸对土壤胶体矿物吸附酸性磷酸酶的影响  

Effect of acetic acid on adsorption of acid phosphatase by some soil colloids and clay minerals.

作　　者：赵振华[1] 黄巧云[2] 蒋新[1] 余贵芬[1] 王芳[1] 李学垣[2] 

机构地区：[1]中国科学院南京土壤研究所,南京210008 [2]华中农业大学资源与环境学院,武汉430070

出　　处：《应用生态学报》2004年第3期482-486,共5页Chinese Journal of Applied Ecology

基　　金：国家重点基础研究发展规划项目 ( 2 0 0 2CB410 80 5 );国家自然科学基金项目 ( 4 0 2 710 64 );中国科学院王宽诚博士后工作奖励基金资助项目

摘　　要：研究了不同 pH值、不同浓度乙酸对酸性磷酸酶在土壤胶体和矿物表面吸附的影响 .结果表明 ,在pH 2～ 8的乙酸体系中 ,酶在胶体矿物表面的最大吸附 pH一般出现在蛋白的等电点和矿物的零电荷点 (PZC)之间 .各土壤胶体和粘粒矿物对酶的吸附量大小顺序为针铁矿 黄棕壤 >砖红壤 >高岭石 >二氧化锰 .乙酸浓度对酶在胶体矿物表面的吸附量和吸附结合能具有较显著影响 ,在 0～ 2 0 0mmol·L-1范围内 ,随着乙酸浓度的增加 ,酶吸附量呈现先升高、后降低、再稳定的趋势 ,而吸附结合能的变化与此相反 .This paper studied the effect of acetic acid with different concentrations and pH values on the adsorption of acid phosphatase by some soil colloids and clay minerals (SCCM).The results showed that the pH values for the maximum adsorption of the enzyme were between the IEP of the enzyme and the PZC of SCCM.In the acetic acid systems,the amount of the enzyme adsorbed by SCCM was in the order of goethiteyellow brown soil>latosol>kaolinite>δ-MnO 2.A remarkable influence of acetic acid concentration on the adsorption amount and the binding energy of the enzyme was observed.With the increase of the concentration from 0 to 200 mmol·L -1 in the system,acetic acid exhibited an enhanced effect,followed by an inhibition action on the adsorption of the enzyme on SCCM.The changes of the binding energy (K value) for the enzyme on SCCM were on the contrary to those of the maximum adsorption.The possible mechanisms for the influence of acetic acid on the adsorption of enzyme by SCCM were also discussed.

关 键 词：土壤胶体矿物 乙酸 酸性磷酸酶 吸附量 吸附结合能 吸附机理 土壤酶 

分 类 号：S153[农业科学—土壤学]