用冷热休克诱导四倍体近江牡蛎  

TETRAPLOIDY INDUCED BY COLD AND HEAT SHOCK IN OSTREA RIVULARIS

作　　者：容寿柏[1] 李一民[1] 刘绍琼[1] 李寿武 范伟 黄雨青 

机构地区：[1]湛江水产学院养殖系

出　　处：《湛江水产学院学报》1992年第2期18-21,共4页

摘　　要：当近江牡蛎授精卵发育至第一次卵裂前3min或个别受精卵开始出现第一次卵裂时,分别用热体克(37℃,39℃,41℃)和冷休克(8℃,10℃,12℃)处理3min诱导四倍体。测定39℃热休克组胚胎早期的四倍体诱导率为28％,10℃冷休克组胚胎早期的四倍体诱导率为30%。温度过高(如42℃以上)可导致卵的高死亡率。When it is 3 minutes before the first cleavage of zygotes of Ostrea rivularis or when one or two eggs begin the first cleavage treat them by heat shocks (37℃, 39℃, 41℃) and cold shocks (8℃, 10℃, 12℃), each lasting 3 minutes to induce tetraploidy.It is measured that the induction frequency of tetrapbidy in the heat shock group(39℃) embry o 's early growth is 28%, and that of the cold-shock group (10℃) is 30%. Too high or too lowa temperature may lead to a high death rate of eggs.

关 键 词：热休克 冷休克 四倍体 牡蛎 

分 类 号：S968.321[农业科学—水产养殖]