人牙源性间充质细胞与陶瓷化骨支架材料相容性的实验研究  

Experimental study on biocompatibility between human dental mesenchymal cells and scaffolds of ceramic bovine bone

作　　者：包柳郁[1] 金岩[2] 史俊南[1] 牛忠英[3] 汪平[1] 赵守亮[1] 王捍国[1] 

机构地区：[1]解放军第四军医大学口腔医学院牙体病科,陕西省西安市710032 [2]解放军第四军医大学口腔医学院病理科,陕西省西安市710032 [3]解放军第三○六医院口腔科,北京市100101

出　　处：《中国临床康复》2004年第8期1458-1459,T003,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家863计划组织工程重大专项资助项目(2002AA205041);国家自然科学基金资助项目(30270374)~~

摘　　要：目的:观察人牙源性间充质细胞在陶瓷化骨表面的贴附、生长和增殖情况,探讨陶瓷化骨作为支架材料应用于构建组织工程牙本质的可行性。方法:组织块法原代培养人牙源性间充质细胞并传代扩增,将经过诱导的牙源性间充质细胞接种于陶瓷化骨三维支架上,通过细胞计数和扫描电镜观察细胞在陶瓷化骨表面的生长和增殖情况。结果:人牙源性间充质细胞在陶瓷化骨表面贴附良好,有较强的增殖能力,扫描电镜见细胞在支架上伸展充分,生长旺盛,细胞表面可见多数分泌颗粒。结论:陶瓷化骨具有良好的生物相容性,可望作为人牙源性间充质细胞的载体用于构建组织工程牙本质的研究。AIM:To observe the adhesion,growth and proliferation of human dental mesenchymal cells on the surface of ceramic bovine bone(CBB),and to explore the feasibility of CBB as scaffolds for tissue engineered dentin. METHODS:Human dental mesenchymal cells were primarily obtained from tissue explant and amplified in vitro.The induced dental mesenchymal cells were seeded into scaffolds of CBB.The growth and proliferation of cells on the surface of CBB were observed through cell counting and scanning electron microscope. RESULTS:Human dental mesenchymal cells adhered and proliferated well on the surface of CBB. Dental mesenchymal cells had a good extension and growth on the surface of CBB,and many secretary particles could be found on the surface of cells under the scanning electronic microscope. CONCLUSION:CBB has good biocompatibility with human dental mesenchymal cells,and can be applied to construct tissue engineered dentin as carriers for dental mesenchymal cells.

关 键 词：人牙源性间充质细胞 陶瓷化骨 支架 生物材料 生物相容性 实验 生物医学工程 

分 类 号：R318[医药卫生—生物医学工程]