黄连素对L-甲状腺素诱发大鼠心肌肥厚的保护作用  

Protective Effect of Berberine on Cardiac Hypertrophy Induced by L-thyroxine in Rats

作　　者：杨静 周祖玉[1] 徐建国[1] 

机构地区：[1]四川大学华西基础医学与法医学院生理学教研室,成都610041

出　　处：《四川大学学报（医学版）》2004年第2期223-225,共3页Journal of Sichuan University(Medical Sciences)

基　　金：四川省科技厅基金 (项目编号 G0 10 6)资助

摘　　要：目的　研究黄连素对 L-甲状腺素 (L- Thy)诱发大鼠发生心肌肥厚的保护作用。方法　 SD大鼠腹腔注射 (ip) L-甲状腺素 0 .5 mg/(kg· d)× 10 d,造成心肌肥厚模型 ,同时用黄连素 30 mg/(kg· d)× 10 d灌胃 ,观察大鼠的心肌肥厚指数 ,心肌细胞膜 Na+ - K+ - ATP酶和肌浆网 Ca2 + - ATP酶活力 ,心肌 NO含量 ,心肌钙调神经磷酸酶 (Ca N)活力的变化。结果　与正常对照组相比 ,心肌肥厚组心肌肥厚指数、Ca N活力明显升高 (P<0 .0 1) ,而心肌NO含量、Na+ - K+ - ATP酶活力和 Ca2 + - ATP酶活力均显著降低 (P<0 .0 1)。黄连素治疗组心肌肥厚指数 ,Ca N活力显著低于肥厚组 (P<0 .0 1) ,心肌 NO含量、Na+ - K+ - ATP酶活力和 Ca2 + - ATP酶活力则显著高于肥厚组 (P<0 .0 1)。结论　黄连素对 L-甲状腺素诱导的大鼠心肌肥厚具有保护作用。Objective To assess the effects of berberine on cardiac hypertrophy inducded by L-thyroxine(L-Thy) in rats. Methods A cardiac hypertrophy model was produced by intraperitoneal (ip) injection of L-thyroxine 0.5 mg/(kg·d)×10 d. 24 SD rats were divided into three groups: ① control (normal saline); ② L-Thy; ③ ip L-Thy + berberine gastrogavage 30 mg/(kg·d)×10 d. Then the cardiac indexes, Na +-K +-ATPase activity, Ca 2+-ATPase activity, the cardiac nitric oxide (NO) content, and the CaN activity were measured. Results Compared with the measurements in the control group, the cardiac indexes and the CaN activity were remarkably increased in the hypertrophy group (P<0.01), but the cardiac NO content, Na +-K +-ATPase activity and Ca 2+-ATPase activity in myocardium were significantly decreased (P<0.01). Further comparison between the hypertrophy group and the L-Thy + berberine group showed that berberine 30 mg/(kg·d)×10 d prevented the ventricular hypertrophy induced by L-Thy and decreased the cardiac indexes and CaN activity (P<0.01); berberine elevated the cardiac NO content, Na +-K +-ATPase activity and Ca 2+-ATPase activity(P<0.01). Conclusion Berberine can prevent the cardiac hypertrophy induced by L-thyroxine in rats.

关 键 词：L-甲状腺素 心肌肥厚 黄连素 保护作用 

分 类 号：R285[医药卫生—中药学]