睾酮的单克隆抗体酶免疫分析法研究  

Studies on the enzymeimmunoassay of testosterone using monoclonal antibody.

作　　者：石放雄[1] 詹勇[1] 傅立平[1] 刘明[2] 金小军[1] 

机构地区：[1]浙江农业大学畜牧兽医系,杭州310029 [2]上海生物制品研究所,上海200025

出　　处：《浙江农业大学学报》1992年第3期88-92,共5页

摘　　要：利用睾酮单克隆抗体,用非平衡法建立鉴定了睾酮的固相微滴板酶免疫分析法,整个测试过程在1小时内完成,结合率(E/E_0)与睾酮浓度的自然对数(InT)之间典型的直线回归方程式为:E/E_0=162.29 InT—19.45(r=-0.9783).用建立的方法测定了海佩科肉鸡59日龄血清睾酮浓度,公鸡为82.08±30.53ng/DL,母鸡为58.84±14.45ng/DL,肉鸡血清睾酮浓度与其体重存在显著的正相关(r=0.4002,n=32).A rapid , simple, solid-phase microtitre plate enzymeimmunoassay ( EIA ) for determination of testosterone using monoclonal antibody was developed by none-equilibrium method. The typical regression equation of dose-response curve was:E/E0 = 162. 29 InT-19. 45(r = -0. 9783). The testosterone concentrations in serume in Haibai Broilers at 59 days-old were determined by the EIA. The results showed : the testosterone concentrations in male and female broiler serums was 82. 08±30. 53 and 58. 84±14. 45 ng/DL, respectively) the testosterone concentrations in serums were closely related with the body weight of the broiler(r = 0. 4002,n = 32).

关 键 词：睾酮 单克隆抗体 酶免疫分析法 

分 类 号：S852.4[农业科学—基础兽医学]