丙基双氢茉莉酮酸酯诱导棉花耐黄萎病的效应  

Biochemical Mechanisms of PDJ-Induced Cotton Resistance to Verticillium wilt

作　　者：何茂华[1] 慕卫[1] 刘峰[1] 

机构地区：[1]山东农业大学植保学院,泰安271018

出　　处：《棉花学报》2004年第2期108-111,共4页Cotton Science

基　　金：山东农业大学博士基金(200115)

摘　　要：温室内以10mg·L 1的丙基双氢茉莉酮酸酯(PDJ)灌根预处理后,再用黄萎病菌(Verticilliumdahliae)孢子悬浮液接种棉苗,处理棉苗与对照相比发病率和病情指数有所降低,表现出对棉花黄萎病的耐病性。对一些生化指标进行测定发现,PDJ灌根和接种都可引起棉苗叶片中苯丙氨酸解氨酶(PAL)活性升高和木质素含量的增加,与此同时棉花叶片中的过氧化物酶(POD)活性及丙二醛(MDA)含量也都有所上升,在此基础上对PDJ诱导棉花耐黄萎病的可能生化机制作了归纳。In greenhouse, the cotton (cv.Simian 3) seedling roots were treated by different concentrations of propyl dihydrojasmonate (PDJ) varying from 1 to 50 mg·L^(-1). Two days after the treatment, cotton gained resistance to cotton Verticillium wilt and the concentration of 10 mg·L^(-1) was more effective. The Phenylalanine amonnia lyase (PAL) activity of cotton seedlings pretreated by 10 mg·L^(-1) PDJ and inoculated with Verticillium dahiliae increased significantly compared with those water-treated within 7 days. Meanwhile, increment of lignin content, Peroxidase (POD) activity and MDA content were also observed in PDJ-treated and inoculated with Verticilluim dahiliae leaves. These changes coincide with the expression of PDJ-induced resistance exactly. Thus, the increment of lignin, MDA content and activities of PAL and POD were considered to be involved in the biochemical mechanisms of PDJ-induced resistance of cotton seedling to Verticillium dahliae.

关 键 词：丙基双氢茉莉酮酸酯 诱导 棉花 耐黄萎病 孢子悬浮液 接种 生理生化机制 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治]