肝气郁结证与脾气虚证的Th细胞分化中的相关性实验研究  

The Experimental Observation of the Relationship between Asthenic Spleen Qi Model and Hepatic Depression Model in Th Cell Activation

作　　者：郑爱华[1] 李家邦[1] 蔡光先[1] 杨冬花 蒋荣鑫[1] 胡学军[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南长沙410008

出　　处：《中国医师杂志》2004年第3期357-359,共3页Journal of Chinese Physician

基　　金：湖南省自然科学基金 (0 1JJY2 1 35)

摘　　要：目的　探讨中医肝脾在T细胞活化中的相关性。方法　应用细胞培养技术和RT -PCR法研究肝郁、脾虚及柴胡疏肝散、四君子汤对应交叉干预后T细胞内IL -4、IFN -r表达的变化。结果　脾虚时IFN -rmRNA表达水平明显低于正常对照组、IL -4rmRNA表达水平明显高于正常对照组 (P <0 0 5 ) ;经四君子汤治疗后IFN -rmRNA表达水平上调、IL -4rmRNA表达水平下调恢复正常 (P <0 0 5 ) ;经柴胡疏肝散治疗后IFN -rmRNA、IL -4rmRNA表达水平无明显变化 ;肝郁时IFN -rmRNA表达水平明显高于正常对照组、IL -4mRNA表达水平明显低于正常对照组 (P <0 0 5 ) ;经柴胡疏肝散治疗后IFN -rmRNA表达水平下调、IL -4mRNA表达水平上调恢复正常 ( P <0 0 5 ) ;经四君子汤治疗后IFN -rmRNA、IL -4mRNA表达水平有所恢复 ,但仍低于正常对照组 ( P >0 0 5 )。Objective To explore relationship between Liver and Spleen of Tradicational Chinse Medicine in T cell activation. Methods The expression of IFN-r and IL-4 of T cells in asthenic spleen qi model and hepatic depression model was detected by RT-PCR. Results IFN-r level of T cells in asthenic spleen qi model significantly decreased, in hepatic depression model significantly increased, in asthenic spleen qi model treated with decoction of four mild drugs rose to normal range, and in hepatic depression model treated with powder of bupleuri dispersing stagnated hepatic qi fell to normal range. The IL-4 level of T cells in asthenic spleen qi model significantly increased, in hepatic depression model significantly decreased, in asthenic spleen qi model treated with decoction of four mild drugs fell to normal range, and in hepatic depression model treated with powder of bupleuri dispersing stagnated hepatic qi rose to normal range. Conclusion Liver and spleen are related to the regulation of THO cells functions.

关 键 词：肝气郁结证 脾气虚证 TH细胞 细胞分化 相关性 柴胡疏肝散 四君子汤 中医药疗法 

分 类 号：R256[医药卫生—中医内科学]