紫色大花矮牵牛组织培养与植株再生  

Tissue culture and plant regeneration of Petunia hybrida

作　　者：权宏[1] 齐莹[1] 施和平[1] 

机构地区：[1]华南师范大学生命科学学院,广东省植物发育工程重点实验室,广东广州510631

出　　处：《亚热带植物科学》2004年第1期51-52,共2页Subtropical Plant Science

基　　金：广东省自然科学基金项目(003062)资助

摘　　要：矮牵牛叶片外植体在MS + 6-BA 1.0mg/L + NAA 0.1mg/L培养基上培养3周后产生致密的浅绿色愈伤组织;转入芽分化培养基MS + 6-BA 0.5mg/L + 4-PU 0.5mg/L + NAA 0.1mg/L 1周后,从愈伤组织表面不断分化产生幼芽;待幼芽长至3cm时转接至生根培养基1/2MS + NAA 1.0 mg/L + GA3 0.5mg/L中生根,长成完整植株。The light green compact callus was developed from leaf explant of Petunia hybrida var. grandiflora when cultured on MS medium supplemented with 6-BA 1.0mg/L + NAA 0.1 mg/L for 3 weeks. When calli were transferred into the differentiation medium, MS + 6-BA 0.5 mg/L + 4-PU 0.5 mg/L + NAA 0.1mg/L for 1 week, adventitious buds were formed. Transferred the buds with 3cm high into the root induction medium (1/2MS+NAA0.1mg/L+GA3 0.5mg/L) for 10d, roots were induced.

关 键 词：矮牵牛 组织培养 植株再生 

分 类 号：Q949.777.7[生物学—植物学] Q943.1