海洋微藻多糖提取纯化条件的研究  

Study on the Conditions of Extracting and Purifying of Polysaccharide from Marine Microalgae

作　　者：吴曼[1] 李文权[1] 张赛金[1] 陈清花[1] 

机构地区：[1]厦门大学海洋学系,亚热带海洋研究所,福建厦门361005

出　　处：《海洋技术》2004年第1期9-12,共4页Ocean Technology

基　　金：国家自然科学基金资助项目 ( 39870 56 5)

摘　　要：研究了提取介质、超声条件和洗脱条件对海洋微藻多糖提取纯化效果的影响。实验结果表明不同 p H的提取介质可以提取得到不同种类的多糖 ,超声条件以 3 0 0 W、 2 0 m in最佳 ,用葡聚糖凝胶 Sephadex-G2 0 0柱 (长 50cm,直径 2 cm)纯化粗多糖 ,当加样量为 4ml,用 0 .2 mol/L NaThe paper investigates the effect of extracting medium, ultrasonic con dition and eluting condition on extraction and purification of polysaccharides f rom marine microalgae. The experimental results indicate that the resulting poly saccharides from marine microalgae are distinct as varied pH in medium. The opti mal ultrasonic power is 300W and the optimal ultrasonic duration is 20min. When the coarse polysaccharides are purified through the Sephadex-G200 column (lengt h: 50cm, diameter: 2cm),the optimal volume of sample added into the column is 4mL, and the optimal eluant is 0 2 mol/L NaCl.

关 键 词：海洋微藻 多糖 提取纯化 洗脱 

分 类 号：P745[天文地球—海洋生物学]