穴位埋药线对癫痫持续状态后神经细胞凋亡的抑制作用  

Effect of Catgut Embedment in Acupoint on c-jun and bcl-2 Expression in Hippocampal Neurons of Rats with Status Epileticus

作　　者：陈文华[1] 庄礼兴[2] 丁晓虹[3] 

机构地区：[1]广州中医药大学解剖教研室,广州510405 [2]广州中医药大学第一附属医院,广州510405 [3]广州军区总医院康复科,广州510010

出　　处：《广州中医药大学学报》2004年第2期118-121,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省中医药管理局基金(编号:111342)

摘　　要：【目的】探讨穴位埋药线法抑制癫痫持续状态(SE)后大鼠海马神经元凋亡的基因水平机制。【方法】以腹腔注射青霉素诱导大鼠SE,采用免疫组织化学法检测SE后大鼠海马神经元凋亡相关基因c-jun、bcl-2的表达。【结果】青霉素致SE后24h,穴位埋药线组、苯妥英钠组和常规针刺组与模型组比较,c-jun表达降低,bcl-2表达升高,两者分别与细胞凋亡指数(Al)呈正、负相关,其中以穴位埋药线组与模型组差异最为显著。【结论】提示穴位埋药线疗法可能通过抑制c-jun、促进bcl-2蛋白表达,阻止SE后大鼠海马神经元凋亡的发生发展,从而起治疗癫痫的作用。[Objective] To explore the genetic mechanism of acupoint catgut embedment in inhibiting hippocampal neuron apoptosis in rats with status epileticus (SE). [Methods] Forty SD rats were randomized into 5 groups: blank control (A), model (B), dilantin (C), routine acupuncture (D) and acupoint catgut embedment (E). Rat models of SE were established by intra-abdominal injection of penicillin. With immunohistochemical method, the expressions of apoptosisrelated genes of c-jun and bcl-2 were observed in the vulnerable neurons of the hippocampus 24 hours after modeling. [Results] Compared with SE model group, the expression of c-jun was decreased and the expression of bcl-2 was increased in group C, D and E 24 hours after modeling; c-jun expression was positively related with the apoptotic index (AI) and bcl-2 expression was negatively related; the effects in group E much differed from those in model group. [Conclusion] The possible mechanism of the acupoint catgut embedment in treating epilepsy is related to the inhibition of the hippocampal neuron apoptosis by prohibiting the expression of c-jun and promoting the expression of bcl-2.

关 键 词：穴位埋药线 癫痫持续状态 神经细胞凋亡 抑制作用 

分 类 号：R245.91[医药卫生—针灸推拿学]