ELISA检测姜片虫病患者抗体的研究  

Studies on detecting antibodies in human seraof Fasciolopsiasis buski by ELISA

作　　者：陈思礼[1] 袁媛[1] 陈强[2] 周雨丝[1] 张喜红[1] 刘辉[1] 

机构地区：[1]华中师范大学生命科学学院,武汉430079 [2]湖北工学院生物工程学院,武汉430068

出　　处：《华中师范大学学报（自然科学版）》2004年第1期85-87,共3页Journal of Central China Normal University：Natural Sciences

基　　金：澳大利亚国家自然科学基金资助项目(ARClargegrant(A)0015010).

摘　　要：研究了姜片吸虫成虫冷浸抗原检测姜片吸虫病患者血清抗体的敏感性和特异性及其在流行病和临床上的应用价值.超声粉碎制备姜片吸虫成虫冷浸抗原.以评价ELISA法检测姜片虫病血清抗体的敏感性、特异性和交叉反应性.按常规ELISA方法操作,目视与酶标仪判断结果.普查2189人中姜片吸虫卵阳性者168例;168例中165例ELISA阳性,ELISA与改良加藤法的阳性符合率98.21%;健康居民血清147份,阳性6份,阳性率4.08%;与血吸虫病交叉阳性为9.38%,肺吸虫病交叉阳性为5.36%.姜片吸虫成虫冷浸抗原1∶3500工作浓度包被酶标板,检测姜片虫病血清抗体具有敏感性高、特异强和交叉反应率低的特点.This paper studied sensitivity, specificity and practical value and significance of ELISA in the detection of antibodies against Fasciolopsis buski in human sera. 168 cases of Fasciolopsiasis were detected for antibodies against Fasciolopsis buski in sera by ELISA, and a comparative investigation was done in the local residents without Fasciolopsis buski and 96 cases of Schistosomiasis japonica and 56 cases of Paragonimus westermani by ELISA for assessment of sensitivity, specificity and crossing reaction. The results were observed with naked eyes and ELISA OD value. Results 165 cases were positive in antibodies against Fasciolopsis buski in human sera among 168 victims of Fasciolopsis buski. The positive correspondence rate between ELISA and Kato-Katz's thick smear was 98.21%. 6 of 147 pieces of sera from the local residents without Fasciolopsis buski was positive in fasciolopsis antibodies. Positive rate was 4.08%. The crossing positive rate to schistosomiasis was 9.38%, to paragonimiasis was 5.36%. Conclusion ELISA with the frozen soaked adult antigen of Fasciolopsis buski to detect fasciolopsis antibodies in human sera had the characteristics of high sensitivity, strong specificity and low crossing reaction rate. It could be used to substitute fecal examination techniques for diagnosis of Fasciolopsis buski.

关 键 词：姜片吸虫 成虫抗原 酶联免疫吸附试验 血清抗体测定 

分 类 号：R383.21[医药卫生—医学寄生虫学]