MC148基因对RANTES趋化单核细胞的拮抗作用  

Study of MC148 gene antagonizing the chemotaxis of monocyte to RANTES

作　　者：孙星慧[1] 吴军[2] 严伟[1] 吴卫真[1] 易绍萱[2] 谭建明[1] 

机构地区：[1]南京军区福州总医院泌尿外科,福建福州350025 [2]第三军医大学西南医院烧伤研究所,重庆400038

出　　处：《免疫学杂志》2004年第2期117-119,共3页Immunological Journal

摘　　要：目的　研究人 β趋化因子同源物MC14 8基因对趋化因子RANTES的拮抗作用。 方法　从传染性软疣病毒 (MCV)基因组中克隆MC14 8DNA片段 ,通过同源重组构建Ad MC14 8腺病毒重组体。重组体转染 2 93细胞 ,从上清液提纯蛋白MC14 8P。通过趋化实验研究MC14 8P对RANTES趋化单核细胞的抑制作用。结果　构建了复制缺陷型腺病毒重组体Ad MC14 8,重组蛋白MC14 8P可以剂量依赖性地拮抗趋化因子RANTES对单核细胞的趋化作用。结论　MC14 8P是趋化因子RANTES的拮抗剂 ,可以拮抗RANTES对单核细胞的趋化作用。Objective To study the antagonism to RANTES of MC148 gene which homo-logous to human β chemokines. Methods MC148 was amplified from DNA of MCV(Molluscum contagiosum virus),isolated and sequenced. The product was then transfected into adenovirus vector to construct recombinant Ad-MC148. MC148P protein was obtained from the supernatant of 293 cells transfected with recombinant Ad-MC148 and served as an antagonist against the recruitment of monocyte to RANTES in chemotaxis essay. Results Recombinant Ad-MC148 was constructed. MC148P antagonized the chemotaxis of monocyte to RANTES in a dose-dependent manner. Conclusion MC148 Can anta- gonize RANTES and inhibit the chemotaxis and recruitment of monocyte to it. MC148 is an antagonist to RANTES. This study might provide a novel way to control immune response.

关 键 词：趋化因子 器官移植 免疫排斥反应 基因克隆 

分 类 号：R392.12[医药卫生—免疫学]