He-Ne激光与丹参注射液联用对牛中性粒细胞呼吸爆发的影响  

Effects of He-Ne Laser Irradiation Combined with Salvia Miltiorrhiza Injection on the Respiratory Burst of Bovine Neutrophils

作　　者：李艳玲[1] 角建瓴[2] 刘承宜[3] 刘颂豪[1] 

机构地区：[1]华南师范大学信息光电子科技学院,广东广州510631 [2]暨南大学中医系,广东广州510632 [3]华南师范大学激光运动医学实验室,广东广州510631

出　　处：《中国激光》2004年第3期381-384,共4页Chinese Journal of Lasers

基　　金：国家自然科学基金 (6 0 1780 0 3和 6 0 2 7812 );广东省自然科学基金团队项目 (2 0 0 0 30 6 1);重点项目 (2 0 0 114 80 );广东省"千百十工程"优秀人才培养基金 (Q0 2 0 87)资助课题

摘　　要：临床上低强度He Ne激光与丹参注射液配合使用治疗缺血、缺氧性心血管疾病取得良好疗效 ,但其机理未明。采用化学发光法研究低强度He Ne激光与丹参注射液联合作用对中性粒细胞 (PMN)呼吸爆发的影响 ,以进一步探讨低强度He -Ne激光与丹参注射液配合使用产生抗缺血、缺氧性损伤效应的机制。实验结果表明低强度He Ne激光具有对受激PMN呼吸爆发的双向调节作用。 2 0 0J/m2 ,2 0 0 0J/m2 和 30 0 0J/m2 的He Ne激光能刺激PMN产生呼吸爆发 ,4 0J/m2 ,10 0 0J/m2 和 6 0 0 0J/m2 的He Ne激光能抑制PMN产生呼吸爆发。 4～ 16 μL的丹参注射液能抑制受激PMN的呼吸爆发 ,且呈一定的量效关系。 8μL的丹参注射液与 10 0 0J/m2 的He Ne激光联合作用能对受激PMN的呼吸爆发产生抑制效应 。He-Ne laser irradiation combined with salvia miltiorrhiza injection (SM) can treat ischemia and anoxemia well in clinic, but the mechanism remains obscure. In this paper, the effects of low intensity He-Ne laser irradiation, SM, and the combination of He-Ne laser irradiation and SM on the respiratory burst of bovine neutrophils were studied, respectively, which may help to understand the mechanism of the combination of He-Ne laser irradiation and SM fight against ischemic injury. The chemiluminescence was used to measure the respiratory burst of neutrophils. The results showed that 200 J/m 2, 2000 J/m 2, 3000 J/m 2 He-Ne laser irradiation can stimulate the respiratory burst of neutrophils, 40 J/m 2, 1000 J/m 2, 6000 J/m 2 He-Ne laser irradiation can inhibit the respiratory burst of neutrophils. 4～16 μL SM can inhibit the respiratory burst of neutrophils, and have the dose-effect relation. The inhibitory effect of the combination of 1000 J/m 2 He-Ne laser irradiation and 8 μL SM is more marked than that of only laser or SM.

关 键 词：心血管病学 低强度HE-NE激光 丹参注射液 中性粒细胞 呼吸爆发 

分 类 号：R54[医药卫生—心血管疾病]