黄瓜子叶节离体再生体系的研究  

In vitro Culture and Plantlet Regeneration from Cotyledonary Nodes of Cucumber(Cucumis sativus L.)

作　　者：赵隽[1] 王华[1] 潘俊松[1] 蔡润[1] 吴爱忠[1,2] 

机构地区：[1]上海交通大学植物科学系 [2]上海市农业科学研究院,上海201106

出　　处：《上海交通大学学报（农业科学版）》2004年第1期43-47,53,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海市科委招标项目(013112006);上海市农委攻关项目(农科攻字(2001)第3-10号)

摘　　要：以6个品系的欧洲温室型全雌系黄瓜子叶节为材料,比较了不同激素组合,苗龄,切割方式和基因型,从而建立了简单快速高效的黄瓜离体再生体系。结果表明:在MS+BA1.5mg·L-1+IAA0.2mg·L-1上启动培养3d为最佳芽启动培养方式,再转入最佳芽诱导培养基MS+TDZ0.02mg·L-1。10d苗龄的子叶节出芽率最高,保留两片子叶各切除1/3为最佳切割处理方式,六种基因型均能生芽,且每外植体出芽数均较高达1.93～3.15。整个过程从种子萌发到驯化移栽,仅需8周。Six European hologynic cultivars of cucumber(Cucumis sativus L.)have been chosen in this experiment. In the test, the cotyledonary nodes were used for the tissue culture to establish a high frequent regeneration system. The results showed that the cotyledonary nodes induced in the medium of MS +BA1.5 mg·L-1 +IAA0.2 mg·L-1 for 3 d was the best and then transferred into the best medium of MS +TDZ0.02 mg·L-1 to induce adventitious shoots and 10 day old cotyledonary node gave rise to high numbers of shoots per explant. The best cutting was to have two cotyledons with 1/3 part remained per cotyledon. Six genotypes could exhibit shoot regeneration and have high numbers of shoots per explant(from 1.93 to 3.15). It only took 8 weeks as a period.

关 键 词：黄瓜 子叶节 离体再生体系 不定芽 再生植株 基因型 切割处理方式 

分 类 号：S642.203.2[农业科学—蔬菜学]