陕西省常见四种实蝇的RAPD研究初报(双翅目:实蝇科)  

Random Amplified Polymorphic DNA in Four Species of Fruit Flies (Diptera: Tephritidae) Distributed Commonly in Shaanxi Province, China

作　　者：张红梅[1] 潘亚勤 魏迪功 仵均祥[1] 

机构地区：[1]西北农林科技大学植保资源与病虫害治理教育部重点实验室,西北农林科技大学植物保护学院,陕西杨凌712100 [2]陕西出入境检验检疫局,陕西西安710068

出　　处：《Entomotaxonomia》2004年第1期59-63,共5页昆虫分类学报（英文）

摘　　要：应用31种随机引物对陕西省常见4种实蝇的基因组DNA进行扩增,筛选出5种引物S61、S107、S126、S275、S1142,可以对4个种扩增出稳定清晰的多态性片段,其中引物S126可以把南瓜实蝇和具条实蝇,具条实蝇和三点棍腹实蝇分开;利用UPGMA法聚类构建的系统树与传统分类完全一致。DNA polymorphisms in 4 species of Tephritidae, distributed commonly in Shaanxi Province, were studied by means of random amplified polymorphic DNA (RAPD). The results show that only 5 primers, which are S61, S107, S126, S275, S1142, could be used to amplify DNA and to obtain stable and clear DNA bands in all of tested 31 primers. B.tau and B. scutellaris, B. scutellaris and D. trimacula could be distinguished each other by primer S126 respectively. The phylogenetic tree constructed by UPGMA was consistent with the conventional phylogenetic relationship among four species.

关 键 词：实蝇 RAPD 分子鉴定 

分 类 号：Q969[生物学—昆虫学]