新台糖25号组织培养及快速繁殖研究  

Studies on rapid propagation for tissue culture of Xintaitang No.25

作　　者：潘一山[1] 罗开梅[1] 邹金美[1] 张聪敏[1] 

机构地区：[1]漳州师范学院生物科学与技术系,福建漳州363000

出　　处：《漳州师范学院学报（自然科学版）》2004年第1期65-67,42,共4页Journal of ZhangZhou Teachers College（Natural Science)

摘　　要：本研究采用新台糖25号蔗株茎尖培养出绿苗和茎梢嫩叶诱导出愈伤组织,再分化出绿苗的方法,快速成功培育出了大批量绿苗.试验表明茎尖培养用MS+6-BA 2.0 mg·L-1+NAA 0.3 mg·L-1附加活性炭0.05%;MS+2,4-D 1.0～3.0 mg·L-1对诱导愈伤组织效果较佳, MS+6-BA 1.0 mg·L-1+KT 1.0 mg·L-1+ NAA 0.3 mg·L-1对分化绿苗及其增殖效果最好;生根培养基选用1/2 MS+IBA 1.0 mg·L-1+NAA 1.0 mg·L-1,附加活性炭0.05%效果好.To propagate rapidly Xintaitang No.25(a new variant of sugarcane), we successfully cultivated substantial seedling of Xintaitang No.25 , using the technique for tissue culture, including the cultivation of fascicular seedling developed from shoot tip and the differentiation of tender seedling after inducing the callus from tender leaf at shoot tip. The shoot tip was cultivated on MS medium with 6-BA 2.0 mg·L-1, NAA 0.3 mg·L-1and appended 0.05% active carbon. The MS medium with 2,4-D 1.0～3.0 mg·L-1 was more effective for callus induction. The MS medium with 6-BA 1.0 mg·L-1, KT 1.0 mg·L-1, NAA 0.3 mg·L-1 was the best choice for the differentiation and reproduction of tender seedling. The root grew best on the 1/2 MS medium with IBA 1.0 mg·L-1, NAA1.0 mg·L-1 and 0.05% active carbon.

关 键 词：新台糖25号 组织培养 快速繁殖 糖料作物 

分 类 号：S566[农业科学—作物学] Q943.1[生物学—植物学]