胃热证动物模型的建立  

Establishment of Animal Model with Stomach-heat Syndrome

作　　者：梁念海[1] 宓穗卿[2] 胡瑞德[3] 王培训[2] 

机构地区：[1]广州中医药大学2000级硕士生,广州510405 [2]广州中医药大学临床药理研究所,广州510405 [3]中山大学基础医学院病理教研室,广州510089

出　　处：《中药新药与临床药理》2004年第2期82-86,共5页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的用SD大鼠建立模拟人胃热证临床症状的实验动物模型,为进一步揭示胃热证内在的生理病理机制研究服务。方法先建立一个较为客观的评定指标,在相对恒定的实验室条件下,用干姜水煎剂作为造模工具药给大鼠灌胃造模,2周后观察其症状体征的变化,组织学检查及生化指标检测,并用“胃热清胶囊”作以药测证的实验研究。结果2周后造模大鼠可见口渴喜饮、舌红等胃热证主要症状体征,胃粘膜弥漫性充血,胃粘膜TXB2、6-keto-PGI2显著上升,符合胃热证动物模型的评定标准;造模大鼠灌服胃热清胶囊后,其胃热证的体征、组织学检查等均有显著效益。结论在符合要求的实验室条件下,对大鼠灌服干姜水煎剂能较成功地建立模拟人胃热证临床症状的大鼠模型。Objective SD rat models of stomach-heat syndrome were established to further investigate the physiopathological mechanism of stomach-heat syndrome.Methods Under certain laboratory condition,criteria of stomach-heat syndrome for the animal models were set up firstly.Then decoction of Rhizoma Zingiberis was given to the rats for modeling.Two weeks after modeling,the symptoms of the rats were observed and pathological and biochemical examination was carried out.Weireqing capsules were used to confirm the success of the establishment of stomach-heat syndrome in rats.Results Two weeks after modeling,the model rats had the symptoms of thirst with preference for drinking and reddened tongue.Congestion in gastric mucosa occurred and the levels of 6-keto-PGF1αand TXB2 in the model group were higher than those in the control group.However,in model rats pretreated with Weireqing,the symptoms and signs of stomach-heat syndrome and histological changes were relieved.Conclusion Under the controlled laboratory condition,feeding rats with decoction of Radix Zingiberis can be successfully used to establish animal model of human stomach-heat syndrome .

关 键 词：胃热证 实验动物模型 胃热清胶囊 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]