HPLC法测定圣济解毒颗粒中黄芩苷的含量  

Determination of Baicalin in Shengjijiedu Granule by HPLC

作　　者：赖荣胜[1] 潘金火[1] 

机构地区：[1]南京中医药大学,南京210029

出　　处：《中药新药与临床药理》2004年第2期118-120,共3页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的采用反相高效液相色谱法测定圣济解毒颗粒中黄芩苷的含量。方法样品用70%乙醇超声提取,色谱柱KromasilC18(200mm×4.6mm,5μm),流动相为甲醇-水-磷酸(50∶50∶0.2),流速0.9mL·min-1,检测波长280nm。结果黄芩苷在0.015～0.121μg范围内线性关系良好,r=0.9999,平均回收率为100.98%,RSD为0.84%。结论该方法灵敏、简便易行、重现性好,可用于该制剂的质量控制。Objective To establish a method for the determination of Baicalin content in Shengjijiedu Granule by HPLC.Method HPLC was performed to determine naringin content on C18 column.The mobile phase consisted of a mixture of CH3OH-H2O-H3PO4(50 ∶50 ∶0.2) and detection wavelength was at 280 nm.Result The linearity of baicalin was in the range of 0.015～0.121 μg (r=0.9999) and the average recovery rate was 100.98 %,RSD=0.84 %.Conclusion This method was simple,sensitive and accurate,and can be used for the quality control of Shengjijiedu granule.

关 键 词：圣济解毒颗粒 黄芩苷 含量测定 HPLC 

分 类 号：R284.1[医药卫生—中药学]