银杏酸凝胶剂的制备及抑菌试验研究  

Studies on Preparation of Ginkgolic Acids Gel and Its Antibacterial Test

作　　者：张小利[1] 欧阳臻[1] 杨小明[1] 陈钧[1] 

机构地区：[1]江苏大学生物与环境工程学院,江苏镇江212013

出　　处：《中药新药与临床药理》2004年第2期124-126,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：江苏省中医药管理局项目(编号H-029)。

摘　　要：目的制备银杏酸凝胶剂,并对该制剂的体外抑菌活性进行初步研究。方法以银杏酸为主药,卡波普为基质制备凝胶剂,采用HPLC法测定制剂中银杏酸含量,并采用液体两倍稀释法测定银杏酸凝胶剂对金黄色葡萄球菌和丙酸杆菌的体外最小抑菌浓度(MIC)。结果银杏酸凝胶剂对金黄色葡萄球菌和丙酸杆菌均显示不同程度的抑菌活性,其MIC分别为31.756mg·mL-1和0.992mg·mL-1。结论该制剂制备工艺可行,性质稳定,对金黄色葡萄球菌和丙酸杆菌有较好的抑制作用。Objective To prepare the preparation of ginkgolic acids gel and to study its antibacterial activity in vitro.Methods The gel was prepared with ginkgolic acids as main component and with Carbopol 940 as base material.The content of ginkgolic acids in the gel was determined by high performance liquid chromatography and the minimum inhibitory concentration (MIC) of the gel against staphylocococus aureus and propionibacteria was measured in vitro by means of liquid-doubling dilution.Results The ginkgolic acids gel has inhibitory effects on staphylocococus aureus and propionibacteria to various extents.And the MIC was 31.756 mg·mL-1 for staphylocococus aureus and 0.992 mg·mL-1 for propionibacteria.Conclusion The preparation of the gel is feasiblye and the the properties of the gel were stable.The ginkgolic acids gel possesses good antibacterial effects on staphylocococus aureus and propionibacteria.

关 键 词：银杏酸 凝胶剂 高效液相色谱法 抑菌试验 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]