用基因枪将GUS基因导入玉米自交系的瞬时表达  

Factors Affecting the Biolistic Transformation of Commercial Corn Inbred Lines in Northeast China

作　　者：袁鹰[1] 刘德璞[1] 郑培和[1] 温刚[1] 王玉民[1] 徐文静[1] 

机构地区：[1]吉林省农业科学院生物技术室,吉林公主岭136100

出　　处：《玉米科学》2004年第1期41-43,共3页Journal of Maize Sciences

摘　　要：用基因枪法将带有GUS报告基因的PDM803转化接种3～5d的东北常用自交系幼胚和已稳定的胚性愈伤组织。经过3～5d恢复培养将转化的受体材料浸于GUS检测液中,结果均检测到蓝色斑点。同时研究了几个影响基因枪转化GUS基因的瞬时表达因素。在其它参数不变的情况下,受体材料预处理的与否、基因枪的射程和氦气压力等都能影响基因枪转化GUS的瞬时表达量。Immature embryos3～5days after inoculation and embryonic calli of commercial corn inbred lines in Northeast China were bombarded and several factors affecting the instant expression of Gus gene were investigat-ed.The result showed the pretreatment of material,bombardment distance and the pressure of helium affected the instant expression when other parameters were set.

关 键 词：玉米 自交系 GUS基因 基因转化 基因枪法 瞬时表达 

分 类 号：S513[农业科学—作物学]