自体内皮细胞移植重建损伤动脉血管内皮的可行性研究  

The feasibility of endothelial reconstruction in the injured arterial wall by autologus endothelial cells transplantation

作　　者：武晓静[1] 黄岚[1] 晋军[1] 赵刚[1] 蒋世忠[1] 张坡[1] 宋明宝[1] 

机构地区：[1]重庆第三军医大学附属新桥医院全军心血管内科中心,400037

出　　处：《中国介入心脏病学杂志》2004年第1期45-47,共3页Chinese Journal of Interventional Cardiology

基　　金：国家自然科学基金资助 ( 3 0 2 70 5 68)

摘　　要：目的　探讨自体内皮细胞移植重建损伤动脉内皮的可行性。方法　 2 0只健康雄性纯种新西兰大白兔行双侧髂股动脉球囊损伤 ,一侧于损伤后立即经球囊导管行自体静脉内皮细胞移植 ,另一侧为培养基对照。其中 10只动物分别于术后 4h、4d处死 ,行扫描电镜检查 ;5只动物 ,先将细胞用荧光标记物标记后 ,再移植入体内 ,4d后进行荧光示踪检查 ;其余 5只动物于术后 4d行Evansblue染色 ,观察血管通透性变化。结果　术后 4h ,对照组见整个内皮层剥脱 ,暴露出内皮下弹力膜及平滑肌 ,细胞移植组则见部分移植内皮已黏附在内皮剥脱血管壁 ,细胞呈圆形 ,但尚未铺展。细胞移植后4d ,移植的内皮已变形 ,铺展在内皮剥脱血管壁 ,大量荧光标记内皮细胞被覆在内皮剥脱血管段的血管内膜 ,对照组损伤血管几乎完全被Evansblue染成蓝色 ,细胞移植组损伤血管则 6 0 %不被Evansblue着染。结论　自体内皮细胞能有效地经球囊导管移植到内皮损伤血管段。Objective To investigate the feasibility of endothelial reconstruction in the injuried arterial wall by autologous endothelial cells transplantation. Methods New Zealand white rabbits (n=20) were subjected to bilateral iliofemoral artery balloon injury, Cultured autologous venous endothelial cells were immediately transplanted by balloon catheter into one vessel, whereas the contralateral artery received the medium only. In 10 rabbits, vessels were harvested 4 hours and 4 days after transplantation for analysis of endothelial coverage by scanning electron microscope (SEM); In 5 rabbits, the cultured endothelial cells were labeled with a fluorescent tracer before their introduction into the injured vessel, and 4 days after transplantation, vessels were harvested to obtain fluorescent imaging of the seeded endothelial cells; Another 5 rabbits were sacrificed 4 days after transplantation for Evans blue staining. Results Four hours after the operation, SEM demostrated that the endothelial layer in control vessels were denuded completely, whereas some round endothelial cells had adhered into the aterial wall in cell transplantation group; four days after cell transplantation, the transplanted cells had attached and spread in the injuried aterial wall by SEM, a number of fluorescent labeling endothelial cells were observed in the endothelial denuded aterial wall, the vessels that received the medium only were stained nearly completely by Evans blue, whereas in those vessels that received cell transplantation, about 60% area were not stained. Conclusion Autologus endothelial cells can be effectively transplanted into the injuried arterial wall by balloon catheter.

关 键 词：自体内皮细胞移植 血管内皮细胞 可行性 动物实验 球囊成形术 血管损伤 

分 类 号：R654.3[医药卫生—外科学]