中华眼镜蛇毒组分F/H对大鼠心肌细胞缺氧-再给氧损伤的作用探讨  

Rearch on the Effect of Fraction F/H from Naja naja atra Venom on Myocytes Anoxia-Reoxygenation Injures in Rats

作　　者：侯力强[1] 何泉华[1] 江中喜[2] 赵路宁[1] 孔天翰[1] 管锦霞[1] 

机构地区：[1]广州医学院蛇毒研究所,广州510182 [2]广州医学院第二附属医院,广州510260

出　　处：《海峡药学》2004年第1期39-41,共3页Strait Pharmaceutical Journal

基　　金：广东省医学科学技术研究基金 (课题号 B19990 75;B2 0 0 0 0 67);广东省自然科学基金博士启动基金 (课题号 9944 0 9)资助

摘　　要：以 L aarse方法建立大鼠心肌细胞缺氧 -再给氧模型 ,缺氧缺糖 60 min,再给氧 1h,探讨组份 F和 H对实验性心肌细胞缺血再灌注的保护作用。实验结果显示 ,缺氧后心肌细胞成活率显著减少 ,心肌释放 LDH含量显著增加 ,细胞膜流动性下降 ,缺氧再给氧后上述各指标改变加剧。组份 F和 H能明显提高心肌细胞成活率 ,减少 L DH的释放 ,增加细胞膜流动性 ,减少心肌细胞膜的损伤 ,实验结论 ,组份 F和 H有明显抗心肌细胞缺氧 -再给氧损伤的作用 ,尤以组份 F作用更显著。According to Laarse's method,the anoxia-reoxygenation model of cultured rats myocardial cells was developed;anoxia and glucose deficiency for 60min followed by reoxygenation and re-exposure to glucose for 1h,in order to research on the protective effect of Fraction F/H isolated from Naja naja atra venom on experimental myocytes anoxia-reoxygenation injuries.The results showed that the survival rate of myocardial cells in the anoxia group was significantly decreased,release of lactate dehydrogenase(LDH)from myocytes was increased and membrane fluidity was decreased,all the changes were much more severe in the anoxia-reoxygenation group.Fraction F/H increased the survival rate of myocardial cells and membrane fluidity,decreased the release of LDH.These results suggested that Fraction F/H may provide obvious protective effects on the anoxia-reoxygenation damages on myocardium.

关 键 词：中华眼镜蛇毒 化学组分 抗氧化 心肌细胞 乳酸脱氢酶 缺氧-再给氧损伤 大鼠 

分 类 号：R996.3[医药卫生—毒理学]