快速老化小鼠海马抑制消减cDNA文库的构建  

Construction of suppression subtracted cDNA library of hippocampus of senescence-accelerated mouse

作　　者：程肖蕊[1] 周文霞[1] 张永祥[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《军事医学科学院院刊》2004年第1期19-21,33,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：国家重点基础研究发展规划项目 (G19990 5 44 0 1)资助

摘　　要：目的 :构建快速老化小鼠 (senescence_acceleratedmouse ,SAM)海马抑制消减cDNA文库。方法 :以SAM快速老化亚系SAM_prone 8(SAMP8)海马作为实验组 ,抗快速老化小鼠亚系SAM_resistance 1(SAMR1)海马作为对照组 ,应用抑制消减杂交 (SSH)技术 ,构建SAMP8海马特异表达cDNA抑制消减文库 ;用蓝白斑筛选 ,以PCR鉴定文库质量。结果 :构建文库的阳性克隆率为 96 .18% ,克隆中cDNA片段介于 2 5 0～ 2 0 0 0bp之间。结论 :成功构建了SAMP8海马抑制消减文库。Objective: To construct the suppression subtracted library of hippocampus of senescence-accelerated mouse (SAM). Methods: Suppression subtractive hybridization (SSH) technique was used to construct the subtracted hippocampal cDNA library of SAM. The hippocampal cDNA of SAMP8 (SAM-prone/8), a senescence-accelerated substrain of SAM,was used as tester, while that of SAMR1 (SAM-resistance/1), a senescence-resistant substrain of SAM,as driver. The subtracted cDNA library was screened by blue/white blot and analysed by PCR. Results: The positive ratio of constructed cDNA library was 96.18% and the length of cDNA fragments ranged from 250 to 2?000?bp. Conclusion: The suppression subtracted cDNA library of hippocampus of SAM is successfully constructed.

关 键 词：快速老化小鼠 海马 抑制消减杂交 基因文库 

分 类 号：R346[医药卫生—基础医学]