芥子气中毒早期肝组织ET-1及ETAR转录表达的研究  

Transcription and expression of endothelin-1 and endothelin A receptor in liver after mustard poisoning

作　　者：曹霞[1] 张黎明[1] 陈志龙[1] 李正银[1] 辜清[2] 

机构地区：[1]第二军医大学海军医学系,上海200433 [2]南昌大学生命科学与食品工程学院,江西南昌330047

出　　处：《军事医学科学院院刊》2004年第1期22-24,共3页Bulletin of the Academy of Military Medical Sciences

基　　金：全军医药卫生"十五"指令课题 ( 0 1L0 5 4)

摘　　要：目的 :探讨芥子气中毒早期肝组织内皮素_1(ET_1)、内皮素A型受体 (ETAR)的变化和意义。方法 :应用免疫组化及RT_PCR技术 ,观测大鼠芥子气中毒后肝组织ET的含量及ET_1,ETARmRNA水平的变化 ,以及该变化与肝组织损伤的关系。结果 :中毒后肝组织ET的含量先下降后增加 ,12h达峰值 ;中毒后肝preproET_1mRNA的表达在 2～ 6h低于对照组 ,12h增加达峰值 ;ETARmRNA的表达没有明显的变化。结论 :ET_1及其受体ETAR在芥子气中毒后肝的病理生理过程中可能起着重要作用。Objective:To explore the role of endothelin-1(ET-1) and endothelin A receptor(ETAR) in pathophysiology of the liver after mustard poisoning.Methods:The changes in transcription and expression of ET-1 and ETAR in the liver and their relationship with the tissue damage were studied by immunohistochemistry and RT-PCR. Results: The level of ET in the liver was significantly decreased 2-12 hours after mustard poisoning, but increased at 12 hours. The transcription and expression of ET-1 was decreased at the early stage after poisoning, but increased at 12 hours. mRNA of endothelin A receptor was unchanged significantly. Conclusion: ET-1 and ETAR may play important roles in the pathophysiological process of the liver after mustard poisoning.

关 键 词：芥子气 中毒 内皮素-1 内皮素受体A 肝 

分 类 号：R595.1[医药卫生—内科学]