反相离子对液相色谱法测定复方降压胶囊中磷酸氯喹含量的研究  

Determination of Chloroquine Phosphate in Compound Hypotensor Capsules by RP Ion-Pair-HPLC

作　　者：翁水旺[1] 

机构地区：[1]福建省药品检验所,福建福州350001

出　　处：《解放军药学学报》2004年第1期69-70,共2页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的　利用反相离子对液相色谱法测定复方降压胶囊中磷酸氯喹的含量。方法　采用HYPERSILODS2 (4 .6× 2 5 0mm ,5 μm)色谱柱 ,乙腈 -甲醇 -己烷磺酸钠溶液 (18∶18∶6 4 )为流动相 ,流速为 1.0ml/min ,检测波长 343nm。 结果　磷酸氯喹线性范围为 :2 1.6～ 2 81μg·ml-1,r=0 .9999;日内精密度 RSD 为 0 .4 2 % (n=6 ) ;日间精密度 RSD 为 0 .5 5 % (n=5 )。平均回收率为97.1% (n =9)。结论　本方法简便 ,准确 。Aim A method was established for the determination of chloroquine phosphate in compound hypotensor capsules by RP-ion-pair-HPLC.Methods The HPLC system consisted of HYPERSIL ODS2 column (4.6×250mm,5μm),mobile phase of acetonitrile-methanol-sodium hexanesulfonate solution (18∶18∶64), with detection at 343nm.Results The linear range of chloroquine phosphate was 21.6～281μg·ml -1,r=0.999 9.The within-day precision RSD was 0.42%(n=6),and the day-to-day precision RSD was 0.55% (n=5).The average recovery was 97.1%(n=9).Conclusion This method was simple and accurate.It was suitable for the preparation quality control.

关 键 词：反相离子对液相色谱法 含量测定 复方降压胶囊 磷酸氯喹 降血压药 

分 类 号：R972.4[医药卫生—药品] R927.2[医药卫生—药学]