条叶龙胆愈伤组织培养及龙胆苦甙形成  

CALLUS CULTURE AND GENTIOPICROSIDE FORMATION IN GENTIANA MANSHURICA KITAG.

作　　者：张治国[1] 韩献忠[1] 蔡志光 刘骅[1] 王黎[1] 赵立红 胡欣[1] 

机构地区：[1]浙江省医学科学院,杭州310013

出　　处：《Acta Botanica Sinica》1992年第2期96-100,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金

摘　　要：应用单因子和正交设计试验,研究了条叶龙胆(Gentiana manshurica Kitag.)愈伤组织生长和龙胆苦甙形成的适宜培养基。结果表明:对于愈伤组织生长,植物激素以 NAA 1mg/L+KT 0.5mg/L为最好,但龙胆苦甙形成的适宜植物激素为NAA1mg/L。B5培养基有利于愈伤组织生长,而龙胆苦甙在MS培养基上形成较多。正交设计试验表明:NH_4^+/NO_3^-,K^+,Ca^(2+)和蔗糖对愈伤组织鲜重、干重,龙胆苦甙含量有显著影响。但愈伤组织生长和龙胆苦甙形成,所要求的因子水平不同。在18组培养基中,7号培养基适合愈伤组织生长,而10号培养基有利于龙胆苦甙的形成。Suitable medium for callus growth and gentiopicroside formation of Gentiana manshurica Kitag. was studied employing orthogonal design and monofactorial expe- riment. The results showed that combination of NAA 1 mg/L + KT 0.5 mg/L was best for callus growth, while NAA 1mg/L. is the suitable phytohormone for genti- opicroside formation. B5 medium was suitable for callus growth while MS medium was suitable for gentiopicroside formation. The experiment of orthogonal design indicates that NH_4^+/NO_3^-, K^+, Ca^(2+) and sucrose were the factors significantly af- fecting fresh weight, dry weight and gentiopicroside content of callus. However, the level of some factors for callus growth was different from that for gentiopicroside formation. In 18 different media, No. 7 was best for callus growth and No. 10 medium was best for gentiopicroside formation.

关 键 词：条叶龙胆 愈伤组织 龙胆苦甙 

分 类 号：S567.01[农业科学—中草药栽培]