绿豆芽中微管蛋白异型的研究  

STUDIES ON ISOTYPE OF TUBULINS IN MUNG BEAN SEEDLINGS

作　　者：刘箭[1] 王秀珍[1] 庞淑敏 

机构地区：[1]北京农业大学生物化学教研室,北京100094

出　　处：《Acta Botanica Sinica》1992年第6期410-415,共6页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金资助的课题

摘　　要：采用 DEAE-Sephadex-A50离子交换层析和 PGGE 电泳对绿豆(Phaseolus radiatus L.)芽中的微管蛋白进行分离。Western blot 和免疫点印迹分析发现了两种聚合度不同的微管蛋白异型。单体微管蛋白亚基的分子量为56kD 和56.5kD;四聚体微管蛋白亚基的分子量为54kD 和54.5kD。实验结果表明微管蛋白α、β亚基组装过程是一个相互选择的过程,这种现象可能与微管蛋白基因表达有关。The tubulin in mung bean(Phaseolus radiatus 1.)seedling was purified by employing DE- AE-A50 ionic exchange column and PGGE.Western blot and immunodot analysis indicated that there are two kinds of isotubulins which have different degree of polymerization.The su- bunit molecular weights of monomer were 56kD and 56.5kD and those of tetramer were 54 kD and 54.5kD.The results suggest that the assembly of α-and β-Subunit is a selective process which might be related to the expression of tubulin genes.

关 键 词：微管 微管蛋白 异型 绿豆 

分 类 号：S522.01[农业科学—作物学]