河西走廊不同生态型芦苇可溶性蛋白质、总氨基酸和游离氨基酸分析  

ANALYSES OF SOLUBLE PROTEIN, TOTAL AND FREE AMINO ACIDS IN LEAVES OF DIFFERENT ECOTYPES OF PHRAGMJTES COMMUNIS GROWING IN THE HEXI CORRIDOR

作　　者：任东涛 张承烈[1] 

机构地区：[1]兰州大学生物系

出　　处：《Acta Botanica Sinica》1992年第9期698-704,共7页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金资助项目

摘　　要：本研究结果证明,分布在甘肃省河西走廊的4种生态型芦苇(Phrogmites communisTrin.),总氨基酸组成中均以天冬氨酸(Asp)、谷氨酸(Glu)、甘氨酸(Gly)、亮氨酸(Leu)和丙氨酸(Ala)含量高,而在17种氨基酸组成中,仅有Ala、苯丙氨酸(Phe)、蛋氨酸(Met)、苏氨酸(Thr)和脯氨酸(Pro)5种组分表现出生态型间的量值差异。游离氨基酸中以Ala、Glu、Asp、Gly和丝氨酸(Ser)为主要成分,但因生态型不同,游离氨基酸组成中各组分在量上的差异显著,其中Pro含量以盐化草甸芦苇最高(为沼泽芦苇的3.5倍),盐化草甸-沙丘过渡地带芦苇和沙丘芦苇次之(为沼泽芦苇的1.6倍)。可溶性蛋白质表现为,沼泽芦苇叶内显示出11种不同分子量的蛋白质组分,其它3种生态型芦苇则显示出13种不同分子量的蛋白质。在主要受到不同程度盐胁迫的3种生态型芦苇叶内出现有66 kD、40.3 kD和16.5kD分子量的所谓盐胁迫蛋白,但其含量与盐胁迫程度不表现正相关。主要受到干旱胁迫的沙丘芦苇叶内含有大量11.7 kD分子量的特异蛋白。这些结果表明,芦苇适应不同生境是以细胞内基因表达产物的改变为基础的,而其耐盐、耐旱性主要与细胞内Pro的累积有关。The difference of total and free amino acids and protein extracted from the leaves of four different reed ecotypes growing in Hexi corridor of Gansu Province were investigated. In all of the different reed ecotypes, the content of Asp, Glu, Gly, Leu and Ala in total amino acids were high, while the contents of Ala, Phe, Met and Thr, Pro in total amino acids varied among different reed ecotypes. Albeit Ala, Glu, Asp, Gly and Ser were the chief composition of free amino acids in leaves of all reed ecotypes, but cemarkble difference was found in the content of each free amino acid from different reed ecotypes. The content of free Pro in leaves of salt meadow and salt meadow-sand dune transitional zone reed were 3.5 and 1.6 times respectively as much as in leaves of swamp reed. Swamp reed had 11 soluble proteins whereas other three reed ecotypes show that each has 13 soluble proteins. Three 'salt adaptation proteins' (66 kD, 40.3 kD, 16.5 kD) were found in leaves of three reed ecotypes with varying degree of salt stress, however, the contents of 3 'salt adaptation protens' showed a negative correlation with the degree of salt stress. There was a large amount of 'special protein' (11.7 kD) in leaves of sand dune reeds. These results suggest that the difference in cytogene expression takes a priority basis of adaptation of reed plants to different habitats, while a closer relationship of reeds tolerance to salt or drought stress with Pro accumulation in cells is seen than with the of accumulation stress adaptation protein.

关 键 词：芦苇 生态型 总氨基酸 可溶性蛋白 

分 类 号：S564.201[农业科学—作物学]