小麦高分子量谷蛋白基因的结构分析  

NUCLEOTIDE SEQUENCE OF A HIGH-MOLECULAR-WEIGHT GLUTENIN GENE IN WHEAT

作　　者：忻骅[1] 江瑛 黄伟达[1] 顾其敏[1] 孙崇荣[1] 

机构地区：[1]复旦大学生物化学系

出　　处：《Acta Botanica Sinica》1992年第10期729-735,共7页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家高技术研究发展计划(863计划)资助项目

摘　　要：应用pUC118/pUC119载体,将一个从小麦(Triticum aestivum L.cv.asarce)基因文库中分离到的含高分子量(HMW)谷蛋白基因的重组子进行了亚克隆,并制作了它们的逐次缺失系列克隆。用Sanger双脱氧末端终止法测定了其中HMW谷蛋白结构基因及编码区上游约560bp的核苷酸顺序,全长3067bp,结构基因由830个密码子组成。基因内部没有内含子,与以前报道的一个小麦HMW谷蛋白基因具有很高的同源性,但其编码区内部却出现了一个TAA终止密码,这是否是一个假基因有待于进一步的研究。Subclones of a wheat (Triticum aestivum L.cv.asarce) genomic recombinant containing high-molecular-weight (HMW) glutenin gene were constructed by using pUC118/pUC119 vectors, their successive shorter deletions were also prepared.The nucleotide sequence of about 560 bp upstream of initiation codon and total coding region was analysed by Sanger's dideo-xynucleotide chain termination method.It has 3067 bp which includes 830 codons and reveals high homology with a previously reported HMW glutenin gene.This gene contains no intron but a TAA termination codon within the coding region.Whether this is a silent gene or not merits further investigation.

关 键 词：小麦 高分子量 谷蛋白基因 结构 

分 类 号：S512.101[农业科学—作物学]