肾动脉性高血压大鼠血清一氧化氮、血管紧张素Ⅱ和超氧阴离子水平的变化  

The changes of serum nitric oxide, angiotensin Ⅱ and superoxide anion in renal artery hypertension rat

作　　者：马向红[1] 杨万松[1] 黄体钢[1] 周丽娟[1] 倪燕平[1] 樊振旺[1] 

机构地区：[1]天津医科大学第二医院心内科,心脏病学研究所,天津市300211

出　　处：《岭南心血管病杂志》2004年第1期57-59,共3页South China Journal of Cardiovascular Diseases

摘　　要：目的　探讨一氧化氮、血管紧张素Ⅱ和超氧阴离子 (O2 -)在高血压发病机制中的作用。方法　将体重2 5 6～ 2 85g雄性Wistar大鼠随机分成 5组 ,每组 10只 ,实验前测量血压、心率。对照组进行假手术及常规饮食喂养 ,单纯结扎组 :不完全结扎一侧肾动脉及常规饮食喂养 ,结扎 +Losartan组 :不完全结扎一侧肾动脉并在水中加入Losartan2 0mg·kg-1·d-1,结扎 +精氨酸 (L Arg)组 :不完全结扎一侧肾动脉并在水中加入L Arg 2g·kg-1·d-1,结扎 +L Arg +Losartan组 :不完全结扎一侧肾动脉并在水中加入L Arg 2g·kg-1·d-1和Losartan 2 0mg·kg-1·d-1,结扎后 1周测量血压、心率并取血测血管紧张素Ⅱ (AngⅡ ) ,环岛苷磷酸 (cGMP) ,一氧化氮 (NO) ,一氧化氮合成酶 (NOS) ,O2 -,超氧化物歧化酶 (SOD)浓度。结果　单纯结扎组收缩压较结扎前明显升高 (P <0 0 5 ) ,而结扎 +Losartan组收缩压较单纯结扎组明显减低 (P <0 0 5 ) ,结扎 +L Arg组血压较对照组增加 ,但较单纯结扎组降低 ,较结扎 +Losartan组高 (P <0 0 5 ) ,结扎 +L Arg +Losartan组血压较对照组增加 ,但低于单纯结扎组和结扎 +L Arg组 (P <0 0 5 )。实验前、后各组心率无变化(P >0 0 5 )。单纯结扎组AngⅡ较对照组明显升高 (P <0 0 5 ) ,而结扎Objectives To study the changes of nitric oxide, angiotensin II and superoxide anion in renal artery hypertension pathogenesis. Methods Male Wistar rats weighing 256～285 g were divided into 5 groups randomly, 10 rats of each group. Control group: false operation was made and routine diet was given; Ligature group: left renal artery was ligatured uncompletely and routine diet was given; Ligature+Losartan group: left renal artery was ligatured uncompletely and Losartan 20 mg·kg -1·d -1 was added in the drinking water; Ligature+L-Arg group: left renal artery was ligatured uncompletely and L-Arg 2g·kg -1·d -1 was added in the drinking water; Ligature+L-Arg+Losartan group: left renal artery was ligatured uncompletely and L-Arg 2g·kg -1·d -1 and Losartan 20 mg·kg -1·d -1 was added in the drinking water. Blood pressure and heart rate were measured before and at the end of the experiment. One week after ligature, blood was drawn to determine angiotensin Ⅱ, cGMP, nitric oxide, nitric oxide synthase(NOS), O - 2, superoxide dismutase(SOD).Results Systolic blood pressure was higher in ligature group than that in control group (P<0.05), systolic blood pressure was much lower in ligature+Losartan group than that in ligature group. Heart rate did not change significantly after experiment (P>0.05). AngⅡ was higher in ligature group than that in control group, even much higher in ligature+Losartan group(P<0.01). There was no difference of cGMP in each group (P>0.05). The concentration of NO was lower in ligature group (P<0.05), NO was higher in ligature+L-Arg+Losartan group than that in ligature group (P<0.05). O - 2 was higher in ligature group and ligature+L-Arg group than that in control group (P<0.05), O - 2 was lower in ligature+Losartan group than that in ligature group (P<0.05). The level of SOD was lower in ligature group than that in control group (P<0.05), higher in ligature+L-Arg group and ligature+L-Arg+Losartan group than that in ligature group (P<0.05). Conclusions A

关 键 词：肾动脉性高血压 大鼠 血清 一氧化氮 血管紧张素Ⅱ 超氧阴离子 发病机制 

分 类 号：R544.14[医药卫生—心血管疾病] R363.1[医药卫生—内科学]