Sensitive Determination of DNA by RLS Enhancement of Metal Ions  

作　　者：Huang Jian-ping 1,2, Chen Fang 1, Ai Xin-ping 1, He Zhi-ke 1 1. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei, China 2. Department of Environmental Engineering, Hubei Agricultural College, Jingzhou 434025, Hubei, China 

出　　处：《Wuhan University Journal of Natural Sciences》2004年第2期234-238,共5页武汉大学学报（自然科学英文版）

基　　金：SupportedbyNationalNaturalScienceFoundationofChina(2 0 2 750 2 8)andtheNationalKeyBasicResearchandDevelopmentProgram (973Program 2 0 0 2CB2 1 1 80 0 )

摘　　要：The interactions between metal ions and DNA have been studied by the resonance light scattering (RLS) spectra. In the acidic condition, the RLS signals of metal ions, especially the transition metal ions in group IB and IIB, were increased by DNA. And it is found that the enhancement of RLS signals is linear to the concentration of DNA, so the RLS method for DNA determination was proposed in the presence of Cu2+. On the optimum conditions, the linear range and the detect limit of ctDNA is 4×10?8–4×10?6 g· mL?1 and 1. 13×10?8 g·mL?1, respectively. The proposed method is successfully applied to determine the extracted plasmid DNA ofBacillus subtilis DB104. Key words resonance light scattering - DNA - metal ions CLC number O 657.3 Foundation item: Supported by National Natural Science Foundation of China (20275028) and the National Key Basic Research and Development Program (973 Program 2002CB211800).Biography: Huang Jian-ping(1964-), male, Associate professor, research direction: bioanalysis.The interactions between metal ions and DNA have been studied by the resonance light scattering (RLS) spectra. In the acidic condition, the RLS signals of metal ions, especially the transition metal ions in group IB and IIB, were increased by DNA. And it is found that the enhancement of RLS signals is linear to the concentration of DNA, so the RLS method for DNA determination was proposed in the presence of Cu2+. On the optimum conditions, the linear range and the detect limit of ctDNA is 4×10?8–4×10?6 g· mL?1 and 1. 13×10?8 g·mL?1, respectively. The proposed method is successfully applied to determine the extracted plasmid DNA ofBacillus subtilis DB104. Key words resonance light scattering - DNA - metal ions CLC number O 657.3 Foundation item: Supported by National Natural Science Foundation of China (20275028) and the National Key Basic Research and Development Program (973 Program 2002CB211800).Biography: Huang Jian-ping(1964-), male, Associate professor, research direction: bioanalysis.

关 键 词：resonance light scattering DNA metal ions 

分 类 号：Q523[生物学—生物化学]