鲁米诺-过氧化氢化学发光体系测定利福平  

Determination of Rifampicin with Luminol-H_2O_2 Chemiluminescence System

作　　者：陈文山[1] 张迎雪[1] 

机构地区：[1]琼州大学环境科学研究所,海南五指山572200

出　　处：《化学研究》2004年第1期37-39,共3页Chemical Research

摘　　要：基于鲁米诺在碱性条件下可以被H2O2氧化产生化学发光,利福平对此化学发光具有增敏作用这一现象,结合流动注射技术建立了一种直接测定利福平的流动注射化学发光新方法.该方法的线性范围在1.0×10-6～2.0×10-8g·mL-1,检出限为3.0×10-9g·mL-1,对5.0×10-7g·mL-1的利福平连续进行11次平行测定,其相对标准偏差为3.0%.该法已成功测定了利福平胶囊及眼药水中利福平的含量.A method to determine rifampicin by flow injection coupled chemiluminescence reaction is developed, based on the chemiluminescence produced during the oxidation of luminol by H_2O_2 in alkaline solution. The chemiluminescence intensity is proportional to rifampicin concentration over the range 1.0×10^(-6)～2.0×10^(-8) g ·mL^(-1 )with a detection limit of 3.0× 10^(-9) g ·mL^(-1). 11 times of parallel determination of 5.0×10^(-7) g ·mL^(-1) rifampicin, show that the relative standard deviation(RSD) is 3.0 %. The method has been sucessfully applied to the determination of rifampicin in capsules and eye drops samples. The proposed method is simple,quick and sensitive.

关 键 词：利福平 抗生素 药物 含量测定 鲁米诺-过氧化氢 化学发光法 流动注射 

分 类 号：R978.1[医药卫生—药品] R927.2[医药卫生—药学]