感染Vero细胞中SARS病原体的电镜观察  

Electron microscopic observation of severe acute respiratory syndrome virus in infected cells

作　　者：朱平[1] 陈洪[2] 陈新宇[1] 陈淑云[2] 杨洁[1] 张岚[1] 易静[1] 刘晶星[2] 

机构地区：[1]上海第二医科大学基础医学院细胞生物学教研室,上海200025 [2]上海第二医科大学基础医学院病原生物学教研室,上海200025

出　　处：《上海第二医科大学学报》2004年第3期192-194,共3页Acta Universitatis Medicinalis Secondae Shanghai

摘　　要：目的 用透射电子显微镜(TEM)观察严重急性呼吸综合征(SARS)患者感染的Vero细胞,为SARS病原体的分离鉴定提供形态学资料。方法 标本为上海市疾病控制中心送检的SARS患者鼻咽拭子接种感染的Vero细胞,对培养液负染色标本和Vero细胞超薄切片标本进行TEM观察。结果 在负染色标本和超薄切片标本中均可观察到病毒,病毒颗粒为圆形或椭圆形,外周有冠状排列的纤突,病毒颗粒直径约80～160 nm,纤突长度约20～40 nm。感染细胞的病毒颗粒分布在细胞质中,主要在内质网和空泡内,可见群集的大小较一致的病毒颗粒。结论 本例电镜所见病毒颗粒大小、形态与文献报道的SARS冠状病毒(SARS-CoV)相符。Objective To utilize electron microscopic study of infected cells to identify the pathogenic agent of severe acute symdrome (SARS). Methods Vero cells were collected with nasopharygeal swabs from SARS patients in Shanghai. The supernatant and infected cells were prepared for electron microscopic study. Results Nagative-stain and ultrathin-seetion revealed the characteristic coronavirus particles. The virus particles of infected cells were in the endoplasmic reticulum and vesicules. They were mainly spherical or oval in shape, 80-160 nm in diameter, with club-shaped surface projections 20-40nm. Conclusion This study indicates a novel coronavirus is the causative agent of the SARS patient.

关 键 词：严重急性呼吸综合征 冠状病毒 电子显微镜 VERO细胞 病原体 细胞形态学 

分 类 号：R446.5[医药卫生—诊断学]