IL-15真核表达质粒的构建及对乙肝疫苗诱导的体液免疫应答的影响  

Cloning of two different IL-15 genes and their effect on the humoral immune response elicited by HBV vaccine

作　　者：张炜[1] 王文逸[1] 陈敏婕[1] 瞿涤[1] 

机构地区：[1]复旦大学上海医学院卫生部分子病毒重点实验室,上海200032

出　　处：《中国免疫学杂志》2004年第3期153-157,共5页Chinese Journal of Immunology

基　　金：国家 973项目 (G19990 5 410 5 );国家自然科学基金项目 (3 0 0 70 693 )

摘　　要：目的 :研究两种不同的IL 15真核表达质粒对乙肝蛋白疫苗诱导的免疫应答的影响。方法 :构建IL 15真核表达质粒 (简称pIL 15 )和含有IL 12信号肽的IL 15真核表达质粒 (简称pIL 2s 15 ) ,CTLL 2细胞增殖实验验证两种质粒真核表达产物的生物学活性。将这两种质粒分别与HBsAg共免疫BALB/C小鼠 ,用ELISA法检测小鼠血清抗 HBsIgG及IgG1、IgG2a亚类的效价。结果 :与HBsAg蛋白疫苗共免疫时 ,pIL 15可使HBsAg诱导的抗 HBsIgG效价升高 ,显著高于载体pcDNA3 1与HB sAg共免疫对照组 ,pIL 2s 15对HBsAg诱导抗 HBsIgG效价没有明显影响。与HBsAg +pcDNA3 1组相比 ,HBsAg +pIL 2s 15组和HBsAg+pIL 15组诱生的抗HBsIgG2a亚类均升高 ,但前者IgG2a/IgG1比值最高 ,与HBsAg +pcDNA3 1组相比差别有显著性 ;HBsAg+pIL 15组IgG2a/IgG1比值与HBsAg +pcDNA3 1组相比差别无显著性。结论 :pIL 15真核表达质粒可增强蛋白疫苗诱导的体液免疫应答 ,pIL 2s 15真核表达质粒则主要使免疫应答趋向Th1型。Objective:To study the effect of IL-15 eukaryotic expression plasmid on the humoral immune responses to HBV surface antigen protein vaccine.Methods:Had constructed two of IL-15 eukaryotic expression plasmides.One is the eukaryotic expression plasmid of the IL-15 whole sequence,the other is the chimeric eukaryotic expression plasmid of the IL-2 signal peptide and IL-15 mature peptide sequence(IL-2s-15).The bioactivities of the expression products of the two plasmids were identified by CTLL-2 proliferation assay.Results:After IL-15 plasmid co-immunized with HBsAg,the titer of anti-HBsIgG was much higher than that of control( P <0.05),but when IL-2s-15 plasmid co-immunized with HBsAg,the titer of anti-HBsIgG was not much different from vector pcDNA3.1 co-immunized with HBsAg( P >0.05),however,the anti-HBsIgG2a/IgG1 ratio was higher than that of control.Conclusion:IL-15 expression plasmids effect differently on the immune responses induced by protein vaccine.

关 键 词：白介素-15 乙肝疫苗 抗体形成 

分 类 号：R510.3[医药卫生—内科学]