不明原因习惯性流产患者Th1/Th2型细胞因子的检测  

Determination of the levels of Th1 and Th2 type cytokines in patients with unexplained habitual abortion

作　　者：董瑞英[1] 许燕雪[1] 江森[1] 

机构地区：[1]山东大学齐鲁医院妇产科,济南250012

出　　处：《中国免疫学杂志》2004年第3期187-189,201,共4页Chinese Journal of Immunology

基　　金：山东省计生委科学研究基金资助 (98 16)

摘　　要：目的 :了解不明原因习惯性流产 (UHA)患者对滋养细胞抗原刺激产生Th1、Th2型细胞因子的水平 ,探讨其在UHA发病中的作用。方法 :选取 5 4例UHA患者 ,35例为未妊娠者 ,为UHA未孕组 ,19例为有UHA病史 ,现在确定妊娠并难免流产者 ,为UHA难免流产组。分别选取 15例正常未孕妇女及正常妊娠者作为对照组。应用酶联免疫吸附法检测未孕各组经滋养细胞抗原刺激的外周血单个核细胞 (PBMC)及妊娠各组PBMC与蜕膜单个核细胞 (DMC)培养上清液中IL 2、IFN γ、IL 4、IL 10的水平。结果 :①在最佳诱导时间下 ,UHA未孕组PBMC产生IL 2、IFN γ的水平明显高于正常未孕组 (P均 <0 0 5 ) ,而IL 4、IL 10水平明显低于正常未孕组 (P均 <0 0 5 )。②UHA难免流产组PBMC及DMC产生的IL 2、IFN γ水平均明显高于正常妊娠组 (P均 <0 0 5 ) ,而IL 4、IL 10水平则明显低于正常妊娠组 ,差异均有显著性 (P均 <0 0 5 )。两组PBMC产生的各细胞因子水平均低于同组DMC ,差异有显著性 (P <0 0 5 )。结论 :UHA患者对滋养细胞抗原产生以Th1型反应为主的免疫应答 ,产生大量Th1型细胞因子 ,导致妊娠丢失。Objective:To determine the levels of Th1 and Th2 type cytokines in patients with unexplained habitual abortion(UHA).Methods:A total of 54 patients with UHA were studied,including 35 cases of nonpregnant woman which were called UNP group and 19 cases of pregnant women with inevitable abortion(IEA) which were called UIEA group.15 cases of normal pregnant(NP) and 15 cases of normal nonpregnant(NNP) women were selected as control groups.Supernatants from trophoblast-activated peripheral blood mononuclear cells(PBMC) of nonpregnant women groups were tested by enzyme-linked immunosorbent assay(ELISA) for interferon gamma(IFN-γ),interleukin-2(IL-2),IL-4,IL-10.Also,these cytokines were tested in the supernatants from PBMC and decidual mononuclear cells(DMC) of pregnant women groups.Results:①The levels of IL-2,IFN-γ in trophoblast-activated PBMC supernatants of UNP group were higher and IL-4,IL-10 were lower than those of normal nonpregnant group( P <0.05).②The levels of IL-2,IFN-γ in PBMC and DMC supernatants of UIEA group were higher and IL-4,IL-10 were lower than those of normal pregnant group( P <0.05).Conclusion:UHA patients have Th1 type immunity to trophoblast and produce high-level Th1 type cytokines which probably result in pregnancy loss.

关 键 词：习惯性流产 TH1细胞 TH2细胞 细胞活素类 

分 类 号：R714.21[医药卫生—妇产科学]