几种胆囊粘膜化学灭活剂的灭活效果评价  

Evaluation of Sclerosing Effects of Several Chemical Sclerosants on Gallbladder Mucosa

作　　者：徐智[1] 彭宣芙[1] 王立新[1] 张能维[1] 邓绍庆[1] 周孝思[1] 

机构地区：[1]北京大学第三医院外科,北京100083

出　　处：《中国普外基础与临床杂志》2004年第2期136-138,共3页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的　研究几种胆囊粘膜化学灭活剂在动物活体的灭活效果。方法　取家兔 2 8只 ,按编号法随机分为 :生理盐水组 (对照组 ) ,95 %酒精组、5 %四环素组和复方苯酚组 (灭活组 )。行胆囊管结扎 ,胆囊造瘘置管并分别注入上述药物留置 15min。 2周后处死动物 ,对胆囊、胆管及胆囊邻近肝脏行病理组织学检查。结果　所有实验动物均存活且活动良好。肉眼观察 ,对照组和 5 %四环素组 7只鼠胆囊腔均无明显变化 ;95 %酒精组中 2只鼠胆囊腔闭塞 ,2只缩小 ,3只无变化 ;复方苯酚组中 ,4只鼠胆囊腔闭塞 ,3只胆囊腔缩小。组织学观察 ,95 %酒精组及复方苯酚组中 7只鼠的胆囊粘膜全部破坏 (7/7) ,与对照组 (1/7)比较差异有显著性意义 (P<0 .0 1) ;而 5 %四环素组有一定效果 (5 /7) ,但与对照组比较差异无显著性意义 (P>0 .0 5 )。结论　 95 %酒精可以作为安全、有效的胆囊粘膜化学灭活剂应用于临床。Objective To evaluate the sclerosing effects of several chemical sclerosants on the gallbadder mucosa in vivo. Methods Twenty-eight rabbits were randomly divided into: Normal saline was used as contrast agent, and 95% ethanol, 5% tetracycline and compound phenol as sclerosants respectively. The cystic duct was ligated and cholecystostomy was performed for each animal. Sclerosants and contrast agent were injected into the gallbladder through a tube respectively and were kept in the gallbladder for 15 minutes. Animals were killed two weeks later; the gallbladder, bile duct and adjacent liver tissue were obtained for histopathological examination. Results With the naked eye, normal saline and 5% tetracycline made the cavity of the gallbladder unchanged; 95% ethanol made two gallbladder cavities occluded, two smaller, and three unchanged; and compound phenol made four gallbladder cavities occluded, and three smaller. In histopathological observations, 95% ethanol and compound phenol could make seven rabbits’ gallbladder mucosa completely destroyed (7/7); compared with normal saline (1/7), there was significant difference (P<0.01). Five percent tetracycline had some effect of ablating the gallbladder mucosa (5/7), but compared with normal saline, there was no difference (P>0.05).Conclusion Ninety-five percent ethanol can be clinically applied as an effective and safe sclerosant.

关 键 词：胆囊粘膜 化学灭活剂 灭活效果 胆囊管 胆囊腔 

分 类 号：R657.4[医药卫生—外科学]