p27在单侧输尿管结扎大鼠肾间质纤维化模型中的表达  

Expression of p27 in renal interstital fibrosis in rats induced by unilateral ureteral obstruction

作　　者：陶立坚 [1] 孙剑 [1] 金鸥 [2] 张军 [1] 沈明 [2] Biao Chen  Damu Tang  李桂源 [4] 

机构地区：[1]中南大学湘雅医院肾内科,长沙,410008 [2]中南大学基础医学院病理科,长沙,410008 [3]Department of Medicine,St.Joseph's Hospital, McMaster University,Canada [4]中南大学湘雅医学院肿瘤研究所,长沙,410078

出　　处：《中南大学学报（医学版）》2004年第1期58-60,共3页Journal of Central South University ：Medical Science

摘　　要：目的 :观察单侧输尿管梗阻 (unilateralureteralobstruction ,UUO)大鼠模型中p2 7在肾小管中的表达 ,了解p2 7与肾间质纤维化程度的关系。方法 :建立UUO大鼠模型 ,采用免疫组织化学方法检测UUO组及假手术组(Sham operatedrates,SOR)术后第 7d ,14d ,2 1d肾小管中p2 7的表达及其与肾间质纤维化程度的关系。结果 :UUO组术后第 7d与SOR组比较 ,p2 7在肾小管上皮细胞中的表达无明显变化 ;术后第 14dUUO组p2 7的表达下降 ,与SOR组比较差异有显著性 (P <0 .0 5 ) ;术后第 2 1dUUO组p2 7的表达明显下降 ,与SOR比较差异有显著性 (P <0 .0 1)。结论 :p2 7在早期UUO大鼠模型中的表达无明显变化 ,随着间质纤维化程度的增加 ,p2Objective To explore the role of p27 in the renal tubule on the process of renal interstitial fibrosis caused by unilateral ureteral obstruction(UUO) in rats. Methods Rats were killed at the 7th, 14th, 21st days after UUO or sham-surgery. The protein expression of p27 in renal tubule was detected by immuohistochemistry at each time point. Results At the 7th day after the surgery, no noticeable abnormalities of p27 expression were found in renal tubular cells between UUO and the sham operated surgery. At the 14th and 21st days after UUO, the p27 expressions in renal tubular cells obviously decreased compared with those after the sham operated surgery. Conclusion The p27 expression has no noticeable changes in the early UUO rats, and then it was significantly reduced with the renal interstitial fibrosis progressing.

关 键 词：P27 输尿管结扎 大鼠 肾间质纤维化 动物模型 免疫组织化学 慢性肾脏疾病 细胞周期 

分 类 号：R692[医药卫生—泌尿科学] R-332[医药卫生—外科学]