通脉汤含药血清对缺氧心肌细胞游离钙及L型钙通道的作用  

Influence of serum contained Tongmai herbal medicine on intracellular calcium concentration and L- type calcium channel in anoxic myocardial cells

作　　者：李彤[1] 孙明[1] 周宏研[1] 龙开超[1] 刘遂心[1] 何飞[1] 

机构地区：[1]中南大学湘雅医院,湖南长沙410008

出　　处：《中国病理生理杂志》2004年第3期391-394,共4页Chinese Journal of Pathophysiology

基　　金：湖南省自然科学基金(No.02jjy3034

摘　　要：目的:研究通脉汤对缺氧心肌细胞钙离子超负荷的保护性作用机制。方法:采用血清药理学方法及建立心肌细胞体外缺氧模型,直接测定不同条件下心肌细胞内的游离钙离子浓度,并通过RT-PCR方法分析细胞膜上L型钙离子通道的表达。结果:在通脉汤含药血清干预下缺氧心肌细胞的游离钙离子浓度显著降低(376.89±78.78nmol/L),达到与左旋氨氯地平相当的水平,并同时降低缺氧条件下的细胞膜L型钙离子通道的表达,而左旋氨氯地平对L型钙通道的表达没有影响。结论:通脉汤可以减轻缺氧心肌细胞钙超负荷,并与钙拮抗剂起到协同作用。AIM: To observe the influence of serum contained Tongmai herbal medicine on calcium overload of the anoxic myocardial cells. METHODS: Serum contained Tongmai herbal medicine was prepared with the serum pharmacological method and the model of anoxic myocardial cells was established. The intracellular free calcium concentration and the expression of the L-type calcium channel were determined with Fura-2/AM and RT-PCR, respectively. RESULTS: The serum contained Tongmai herbal medicine and levo-anlodipine decreased the intracellular free calcium concentration in the anoxic myocardial cells. The serum contained Tongmai herbal medicine also suppressed the expression of L-type calcium channel in the anoxic myocardial cells, but levo-anlodipine had no effect on the expression of L-type calcium channel. CONCLUSION: Tongmai herbal medicine can attenuate calcium overload in anoxic myocardial cells.

关 键 词：心肌 缺氧 钙超负荷 通脉汤 

分 类 号：R541.4[医药卫生—心血管疾病]